Structure of PDB 8prv Chain G

Receptor sequence

>8prvG (length=2034) Species: 4932 (Saccharomyces cerevisiae)

STRPLTLSHGSLEHVLLVPTASFFIASQLQEQFNKILPEPTEGFAADDEP
TTPAELVGKFLGYVSSLVEPSKVGQFDQVLNLCLTEFENCYLEGNDIHAL
AAKLLQENDTTLVKTKELIKNYITARIMAKRPFDKKSNSALFRAVGEGNA
QLVAIFGGQGNTDDYFEELRDLYQTYHVLVGDLIKFSAETLSELIRTTLD
AEKVFTQGLNILEWLENPSNTPDKDYLLSIPISCPLIGVIQLAHYVVTAK
LLGFTPGELRSYLKGATGHSQGLVTAVAIAETDSWESFFVSVRKAITVLF
FIGVRCYEAYPNTSLPPSILEDSLENNEGVPSPMLSISNLTQEQVQDYVN
KTNSHLPAGKQVEISLVNGAKNLVVSGPPQSLYGLNLTLRKAKAPSGLDQ
SRIPFSERKLKFSNRFLPVASPFHSHLLVPASDLINKDLVKNNVSFNAKD
IQIPVYDTFDGSDLRVLSGSISERIVDCIIRLPVKWETTTQFKATHILDF
GPGGASGLGVLTHRNKDGTGVRVIVAGTLDINPDDDYGFKQEIFDVTSNG
LKKNPNWLEEYHPKLIKNKSGKIFVETKFSKLIGRPPLLVPGMTPCTVSP
DFVAATTNAGYTIELAGGGYFSAAGMTAAIDSVVSQIEKGSTFGINLIYV
NPFMLQWGIPLIKELRSKGYPIQFLTIGAGVPSLEVASEYIETLGLKYLG
LKPGSIDAISQVINIAKAHPNFPIALQWTGGRGGGHHSFEDAHTPMLQMY
SKIRRHPNIMLIFGSGFGSADDTYPYLTGEWSTKFDYPPMPFDGFLFGSR
VMIAKEVKTSPDAKKCIAACTGVPDDKWEQTYKKPTGGIVTVRSEMGEPI
HKIATRGVMLWKEFDETIFNLPKNKLVPTLEAKRDYIISRLNADFQKPWF
ATVNGQARDLATMTYEEVAKRLVELMFIRSTNSWFDVTWRTFTGDFLRRV
EERFTKSKTLSLIQSYSLLDKPDEAIEKVFNAYPAAREQFLNAQDIDHFL
SMCQNPMQKPVPFVPVLDRRFEIFFKKDSLWQSEHLEAVVDQDVQRTCIL
HGPVAAQFTKVIDEPIKSIMDGIHDGHIKKLLHQYYGDDESKIPAVEYFG
GESPVEDSAVFKATSSTDEESWFKALAGSEINWRHASFLCSFITQDKMFV
SNPIRKVFKPSQGMVVEISNGNTSSKTVVTLSEPVQGELKPTVILKLLKE
NIIQMEMIENRTMDGKPVSLPLLYNFNPDNGFAPISEVMEDRNQRIKEMY
WKLWIDEPFNLDFDPRDVIKGKDFEITAKEVYDFTHAVGNNCEDFVSRPD
RTMLAPMDFAIVVGWRAIIKAIFPNTVDGDLLKLVHLSNGYKMIPGAKPL
QVGDVVSTTAVIESVVNQPTGKIVDVVGTLSRNGKPVMEVTSSFFYRGNY
TDFENTFQKTVEPVYQMHIKTSKDIAVLRSKEWFQLDDEDFDLLNKTLTF
ETETEVTFKNANIFSSVKCFGPIKVELPTKETVEIGIVDYEAGASHGNPV
VDFLKRNGSTLEQKVNLENPIPIAVLDSYTPSTNEPYARVSGDLNPIHVS
RHFASYANLPGTITHGMFSSASVRALIENWAADSVSSRVRGYTCQFVDMV
LPNTALKTSIQHVGMINGRKLIKFETRNEDDVVVLTGEAEIEQPVTTFVF
TGQGSQEQGMGMDLYKTSKAAQDVWNRADNHFKDTYGFSILDIVINNPVN
LTIHFGGEKGKRIRENYSAMIFETIVDGKLKTEKIFKEINEHSTSYTFRS
EKGLLSATQFTQPALTLMEKAAFEDLKSKGLIPADATFAGHSLGEYAALA
SLADVMSIESLVEVVFYRGMTMQVAVPRDELGRSNYGMIAINPGRVAASF
SQEALQYVVERVGKRTGWLVEIVNYNVENQQYVAAGDLRALDTVTNVLNF
IKLQKIDIIELQKSLSLEEVEGHLFEIIDEASKKSAVKPRPLKLERGFAC
IPLVGISVPFHSTYLMNGVKPFKSFLKKNIIKENVKVARLAGKYIPNLTA
KPFQVTKEYFQDVYDLTGSEPIKEIIDNWEKYEQ

3D structure

• PDB: 8prv Reconstruction of a fatty acid synthesis cycle from acyl carrier protein and cofactor structural snapshots.
• Chain: G
• Resolution: 2.9 Å

Enzymatic activity

• Enzyme Commision number: 1.3.1.9: enoyl-[acyl-carrier-protein] reductase (NADH).
  2.3.1.38: [acyl-carrier-protein] S-acetyltransferase.
  2.3.1.39: [acyl-carrier-protein] S-malonyltransferase.
  2.3.1.86: fatty-acyl-CoA synthase system.
  3.1.2.14: oleoyl-[acyl-carrier-protein] hydrolase.
  4.2.1.59: 3-hydroxyacyl-[acyl-carrier-protein] dehydratase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FMN	G	P595 G596 M597 T598 N650 K706 G737 G738 G770 G802 S803 L1054	P591 G592 M593 T594 N646 K702 G733 G734 G766 G798 S799 L1050
BS02	NAP	G	F625 H740 P1014 K1031 D1032	F621 H736 P1010 K1027 D1028
BS03	COA	G	X1808 M1854 I1857 N1858 R1861 N1890 N1892 Q1897 R1962 A1965 C1966 I1967 L1969	X1792 M1838 I1841 N1842 R1845 N1874 N1876 Q1881 R1946 A1949 C1950 I1951 L1953

Gene Ontology

Molecular Function

• GO:0004312 fatty acid synthase activity
• GO:0004313 [acyl-carrier-protein] S-acetyltransferase activity
• GO:0004314 [acyl-carrier-protein] S-malonyltransferase activity
• GO:0004318 enoyl-[acyl-carrier-protein] reductase (NADH) activity
• GO:0004321 fatty-acyl-CoA synthase activity
• GO:0005515 protein binding
• GO:0016297 fatty acyl-[ACP] hydrolase activity
• GO:0016409 palmitoyltransferase activity
• GO:0016491 oxidoreductase activity
• GO:0016740 transferase activity
• GO:0016787 hydrolase activity
• GO:0016829 lyase activity
• GO:0019171 (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
• GO:0141148 enoyl-[acyl-carrier-protein] reductase (NADPH) activity

Biological Process

• GO:0006633 fatty acid biosynthetic process
• GO:0042759 long-chain fatty acid biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005739 mitochondrion
• GO:0005811 lipid droplet
• GO:0005829 cytosol
• GO:0005835 fatty acid synthase complex

External links

• PDB: RCSB:8prv, PDBe:8prv, PDBj:8prv
• PDBsum: 8prv
• PubMed: 37949058
• UniProt: P07149|FAS1_YEAST Fatty acid synthase subunit beta (Gene Name=FAS1)