Structure of PDB 8e9i Chain G

Receptor sequence

>8e9iG (length=780) Species: 246196 (Mycolicibacterium smegmatis MC2 155)

VEMVSLTIDDHEISVPKGTLLIRAAELMGIQIPRFCDHPLLDPVGACRQC
LVEVEGQRKPMASCTTTVMPDMVVRTQFTSEAADKAQRGVMELLLINHPL
DCPICDKGGECPLQNQAMSNGRPETRFEDVKRTFPKPISISSQVLLDRER
CVLCARCTRFSSQIAGDPFIDLMERGALQQVGIGQDKPFQSYFSGNTVQI
CPVGALTGTAYRFRARPFDLVSSPSVCEHCASGCAQRTDHRRGKVLRRLA
GDEPEVNEEWNCDKGRWAFTYATVGDRITTPMLRDGGVLRPASWSEALTV
AAAGLLTAAGSTGVLVGGRCTVEDAYAYAKFARMVLNTNDVDFRARPHSA
EEAEFLAAHVAGQTMGLRYAELENAPTVLLAGFEPEEESPIVFLRLRKGV
RKNGVQVVAVAPWASRGLTKLAGTVVPTVPGDEPAALDGMHDDDRLRRPG
AVILVGERLATSPGALSAAVRLAAATGARLAWIPRRAGERGAIEAGALPN
LLPGGRPVDDADARAEVARAWYISALPEAPGRDTAAILSTAASGHLAALL
VGGVELGDLPDPELAVAAVRTTPFVVSLELRESAVTELADVVFPVAPVVE
KAGSFLNWEGRPRPFAPSLKTNAIPDLRVLHYLADEIGVDLALPTAEAAD
AELAQLGTWGGARPPAPTAPPTARPEAGSGQAVLASWRMLLDAGRLQDGE
PHLAGTAVRPVARMSAATAAGIGASDGAPVTVSTERGAVTLPLAVTDMPD
GVVWLPMNSPGSAVHQRLGVTAGAVVSIGA

3D structure

• PDB: 8e9i Structure of mycobacterial respiratory complex I.
• Chain: G
• Resolution: 2.8 Å

Enzymatic activity

• Enzyme Commision number: 7.1.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FES	G	R46 C48 D49 G57 C59 R60 Q61 C62 C76	R34 C36 D37 G45 C47 R48 Q49 C50 C64
BS02	GTP	G	R331 R497 R498 G565 E567 L590 R593 V607 R700 G706 R707 L708	R319 R485 R486 G553 E555 L578 R581 V595 R688 G694 R695 L696
BS03	SF4	G	H110 D113 C114 C117 G120 C123 L125 Q126 V215 G216	H98 D101 C102 C105 G108 C111 L113 Q114 V203 G204
BS04	SF4	G	C163 V164 L165 C166 A167 R168 C169 C213 P214 A217 L218	C151 V152 L153 C154 A155 R156 C157 C201 P202 A205 L206
BS05	SF4	G	C239 C242 S244 C246 N273 C274 G277 P402 I403	C227 C230 S232 C234 N261 C262 G265 P390 I391

Gene Ontology

Molecular Function

• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0016491 oxidoreductase activity
• GO:0016651 oxidoreductase activity, acting on NAD(P)H
• GO:0043546 molybdopterin cofactor binding
• GO:0046872 metal ion binding
• GO:0048038 quinone binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051537 2 iron, 2 sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0042773 ATP synthesis coupled electron transport
• GO:1902600 proton transmembrane transport

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:8e9i, PDBe:8e9i, PDBj:8e9i
• PDBsum: 8e9i
• PubMed: 36952383
• UniProt: A0QU30