Structure of PDB 8das Chain G

Receptor sequence
>8dasG (length=474) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]
RYGPLRIKELAVDEELEKEDGLIPRQKSKLCKHGDRGMCEYCSPLPPWDK
EYHEKNKIKHISFHSYLKKLNENANKKENGSSYISPLSEPDFRINKRCHN
GHEPWPRGICSKCQPSAITLQQQEFRMVDHVEFQKSEIINEFIQAWRYTG
MQRFGYMYGSYSKYDNTPLGIKAVVEAIYEPPQHDEQDGLTMDVEQVKNE
MLQIDRQAQEMGLSRIGLIFTDLSDAGAGDGSVFCKRHKDSFFLSSLEVI
MAARHQTRHPNVSKYSEQGFFSSKFVTCVISGNLEGEIDISSYQVSTEAE
ALVTADMISGSTFPSMAYINDTTDERYVPEIFYMKSNEYGITVKENAKPA
FPVDYLLVTLTHGFPNTDTETNSKFVSSTGFPWSNRQAMGQSQDYQELKK
YLFNVASSGDFNLLHEKISNFHLLLYINSLQILSPDEWKLLIESAVKNEW
EESLLKLVSSAGWQTLVMILQESG
3D structure
PDB8das SUMO enhances unfolding of SUMO-polyubiquitin-modified substrates by the Ufd1/Npl4/Cdc48 complex.
ChainG
Resolution3.5 Å
3D
structure
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Enzymatic activity
Enzyme Commision number ?
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN G C137 H139 C145 C31 H33 C39
BS02 ZN G C204 H208 C219 C98 H102 C113
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0031625 ubiquitin protein ligase binding
GO:0036435 K48-linked polyubiquitin modification-dependent protein binding
GO:0043130 ubiquitin binding
Biological Process
GO:0006274 DNA replication termination
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0006515 protein quality control for misfolded or incompletely synthesized proteins
GO:0015031 protein transport
GO:0030970 retrograde protein transport, ER to cytosol
GO:0036503 ERAD pathway
GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0051028 mRNA transport
GO:0051228 mitotic spindle disassembly
GO:0070651 nonfunctional rRNA decay
GO:0071629 cytoplasm protein quality control by the ubiquitin-proteasome system
GO:0072344 rescue of stalled ribosome
GO:0072665 protein localization to vacuole
GO:0072671 mitochondria-associated ubiquitin-dependent protein catabolic process
GO:0099638 endosome to plasma membrane protein transport
GO:1900182 positive regulation of protein localization to nucleus
GO:1990116 ribosome-associated ubiquitin-dependent protein catabolic process
Cellular Component
GO:0000837 Doa10p ubiquitin ligase complex
GO:0000839 Hrd1p ubiquitin ligase ERAD-L complex
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0016020 membrane
GO:0030894 replisome
GO:0031965 nuclear membrane
GO:0034098 VCP-NPL4-UFD1 AAA ATPase complex
GO:0036266 Cdc48p-Npl4p-Vms1p AAA ATPase complex
GO:0042175 nuclear outer membrane-endoplasmic reticulum membrane network
GO:0048471 perinuclear region of cytoplasm
GO:1990112 RQC complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8das, PDBe:8das, PDBj:8das
PDBsum8das
PubMed36574706
UniProtP33755|NPL4_YEAST Nuclear protein localization protein 4 (Gene Name=NPL4)

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