Structure of PDB 8bed Chain G

Receptor sequence

>8bedG (length=687) Species: 3702 (Arabidopsis thaliana)

RNPVGGARVHFSNPEDAIEVFVDGYAVKVPKGFTVLQACEVAGVDIPRFC
YHSRLSIAGNCRMCLVEVEKSPKPVASCAMPALPGMKIKTDTPIAKKARE
GVMEFLLMNHPLDCPICDQGGECDLQDQSMAFGSDRGRFTEMKRSVVDKN
LGPLVKTVMTRCIQCTRCVRFASEVAGVQDLGILGRGSGEEIGTYVEKLM
TSELSGNVIDICPVGALTSKPFAFKARNWELKATETIDVSDAVGSNIRVD
SRGPEVMRIIPRLNEDINEEWISDKTRFCYDGLKRQRLSDPMIRDSDGRF
KAVSWRDALAVVGDIIHQVKPDEIVGVAGQLSDAESMMVLKDFVNRMGSD
NVWCEGTAAGVDADLRYSYLMNTSISGLENADLFLLIGTQPRVEAAMVNA
RICKTVRASNAKVGYVGPPAEFNYDCKHLGTGPDTLKEIAEGRHPFCTAL
KNAKNPAIIVGAGLFNRTDKNAILSSVESIAQANNVVRPDWNGLNFLLQY
AAQAAALDLGLIQQSAKALESAKFVYLMGADDVNVDKIPKDAFVVYQGHH
GDKAVYRANVILPASAFTEKEGTYENTEGFTQQTVPAVPTVGDARDDWKI
VRALSEVSGVKLPYNSIEGVRSRIKSVAPNLVHTDEREPAAFGPSLKPEC
KEAMSTTPFQTVVENFYMTNSITRASKIMAQCSAVLL

3D structure

• PDB: 8bed Cryo-EM structure of the respiratory I + III 2 supercomplex from Arabidopsis thaliana at 2 angstrom resolution.
• Chain: G
• Resolution: 2.03 Å

Enzymatic activity

• Enzyme Commision number: 7.1.1.2: NADH:ubiquinone reductase (H(+)-translocating).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	G	I172 F280	I116 F224
BS02	peptide	G	W361 R362 P645 T646 R651 D652 K655 I656 R658 A659 E662	W305 R306 P589 T590 R595 D596 K599 I600 R602 A603 E606
BS03	FES	G	R104 C106 Y107 G115 C117 R118 C120 C134	R48 C50 Y51 G59 C61 R62 C64 C78
BS04	SF4	G	H166 D169 C170 C173 Q175 G176 C179 L181 Q182 V270	H110 D113 C114 C117 Q119 G120 C123 L125 Q126 V214
BS05	SF4	G	C218 I219 Q220 C221 T222 C224 I248 C268 A272 L273	C162 I163 Q164 C165 T166 C168 I192 C212 A216 L217

Gene Ontology

Molecular Function

• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0016491 oxidoreductase activity
• GO:0016651 oxidoreductase activity, acting on NAD(P)H
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051537 2 iron, 2 sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0009853 photorespiration
• GO:0042773 ATP synthesis coupled electron transport
• GO:1902600 proton transmembrane transport

Cellular Component

• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane
• GO:0009507 chloroplast
• GO:0009536 plastid
• GO:0016020 membrane
• GO:0031966 mitochondrial membrane
• GO:0045271 respiratory chain complex I

External links

• PDB: RCSB:8bed, PDBe:8bed, PDBj:8bed
• PDBsum: 8bed
• PubMed: 36585502
• UniProt: Q9FGI6|NDUS1_ARATH NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial (Gene Name=EMB1467)