Structure of PDB 7u3b Chain G

Receptor sequence

>7u3bG (length=693) Species: 54571 (Streptomyces venezuelae)

QVWPGQAYPLGATYDGAGTNFAVFSEAAHRIELCLLHDDGSETAVELRET
DAFVRHAYLPGVMPGQRYGFRVHGPYAPERGLRCNAAKLLLDPYARAVSG
RVRWGEAVYGYPFGRPDARNDLDSAPDTMTSVVVNPYFDWGDDRRPRTEY
HHTVIYEAHVKGLTMLHPDLPEELRGTYAGLAHPSVIGHLRELGVTALEL
MPVHQFVNDHRLVDAGLSNYWGYNTIGFFAPHNAYASWGDRGQQVLEFKS
AVRALHQAGIEVILDVVYNHTAEGNHLGPTLSMRGLDNPSYYRLADDPRY
YMDTTGTGNSLLMRSPHVLQLIMDSLRYWVTEMHVDGFRFDLAATLARQF
HEVDRLSSFFDLVQQDPVVSQVKLIAEPWDVGEGGYQVGNFPPLWTEWNG
KYRDCVRDLWRGEPRTLAEFASRLTGSSDLYQDDGRRPLASVNFVTCHDG
FTLRDLVSYNEKRNEANGEGNRDGENYNRSWNCGEEGETEDVGITELRAR
QMRNFLATLMLSQGVPMLSHGDEFGRTQGGNNNAYCQDNEVSWVRWPSEA
EATLLRFTRSMVRLRREHPVFRRRRFFHGRPVLTDIAWFTPEGEEMTSRD
WQAAHAQALTVFLNGNAISEPGTQGERIADDSFLLMFNASAKELEFVVPD
SRYWRMVVDTSDPEGMPPQQGPELAGGERVTLAPLSLTVLRRP

3D structure

• PDB: 7u3b Allosteric regulation of glycogen breakdown by the second messenger cyclic di-GMP.
• Chain: G
• Resolution: 3.6 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	A16	G	R212 W222 Y224 G307 D342 L343 E378 H449 N534 Y536	R211 W221 Y223 G306 D341 L342 E377 H448 N533 Y535
BS02	C2E	G	E47 R49 E50 T51 R56	E46 R48 E49 T50 R55
BS03	C2E	G	Q433 R438 R579 F580	Q432 R437 R575 F576

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004133 glycogen debranching enzyme activity
• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0005980 glycogen catabolic process

External links

• PDB: RCSB:7u3b, PDBe:7u3b, PDBj:7u3b
• PDBsum: 7u3b
• PubMed: 36192422
• UniProt: A0A5P2ALW6