Structure of PDB 7tzc Chain G

Receptor sequence

>7tzcG (length=4404) Species: 9986 (Oryctolagus cuniculus)

EVQFLRTDDEVVLQCSATVLKEQLKLCLAAEGFGNRLCFLEPTSNAQNVP
PDLAICCFTLEQSLSVRALQEMLANHRTLLYGHAILLRHAHSRMYLSCLT
TSRSMTDKLAFDVGLQEDATGEACWWTMHPASKQRSEGEKVRVGDDLILV
SVSSERYLHLSTASGELQVDASFMQTLWNMNPICSCCEEGYVTGGHVLRL
FHGHMDECLTISAADSDDQRRLVYYEGGAVCTHARSLWRLEPLRISWSGS
HLRWGQPLRIRHVTTGRYLALTEDQGLVVVDACKAHTKATSFCFRVSKEK
LDTAPKRDVEGMGPPEIKYGESLCFVQHVASGLWLTYAAPDPKALRLGVL
KKKAILHQEGHMDDALFLTRCQQEESQAARMIHSTAGLYNQFIKGLDSFS
GKPRGSGPPAGPALPIEAVILSLQDLIGYFEPPSEELQHEEKQSKLRSLR
NRQSLFQEEGMLSLVLNCIDRLNVYTTAAHFAEYAGEEAAESWKEIVNLL
YELLASLIRGNRANCALFSTNLDWVVSKLDRLEASSGILEVLYCVLIESP
EVLNIIQENHIKSIISLLDKHGRNHKVLDVLCSLCVCNGVAVRSNQDLIT
ENLLPGRELLLQTNLINYVTSIRPNIFVGRAEGSTQYGKWYFEVMVDEVV
PFLTAQATHLRVGWALTEGYSPYPGGGEGWGGNGVGDDLYSYGFDGLHLW
TGHVARPVTSPGQHLLAPEDVVSCCLDLSVPSISFRINGCPVQGVFEAFN
LDGLFFPVVSFSAGVKVRFLLGGRHGEFKFLPPPGYAPCHEAVLPRERLR
LEPIKEYRREGPRGPHLVGPSRCLSHTDFVPCPVDTVQIVLPPHLERIRE
KLAENIHELWALTRIEQGWTYGPVRDDNKRLHPCLVNFHSLPEPERNYNL
QMSGETLKTLLALGCHVGMADEKAEDNLKKTKLPKTYMMSNGYKPAPLDL
SHVRLTPAQTTLVDRLAENGHNVWARDRVAQGWSYSAVQDIPARRNPRLV
PYRLLDEATKRSNRDSLCQAVRTLLGYGYNIEPPDQEPSQVENQSRWDRV
RIFRAEKSYTVQSGRWYFEFEAVTTGEMRVGWARPELRPDVELGADELAY
VFNGHRGQRWHLGSEPFGRPWQSGDVVGCMIDLTENTIIFTLNGEVLMSD
SGSETAFREIEIGDGFLPVCSLGPGQVGHLNLGQDVSSLRFFAICGLQEG
FEPFAINMQRPVTTWFSKSLPQFEPVPPEHPHYEVARMDGTVDTPPCLRL
AHRTWGSQNSLVEMLFLRLSLPVQFHQHFRCTDNRDDPEIILNTTTYYYS
VRVFAGQEPSCVWVGWVTPDYHQHDMNFDLSKVRAVTVTMGDEQGNVHSS
LKCSNCYMVWGGDFVSPGQQGRISHTDLVIGCLVDLATGLMTFTANGKES
NTFFQVEPNTKLFPAVFVLPTHQNVIQFELGKQKNIMPLSAAMFLSERKN
PAPQCPPRLEVQMLMPVSWSRMPNHFLQVETRRAGERLGWAVQCQDPLTM
MALHIPEENRCMDILELSERLDLQRFHSHTLRLYRAVCALGNNRVAHALC
SHVDQAQLLHALEDAHLPGPLRAGYYDLLISIHLESACRSRRSMLSEYIV
PLTPETRAITLFPPGRKGGNARRHGLPGVGVTTSLRPPHHFSPPCFVAAL
PAAGVAEAPARLSPAIPLEALRDKALRMLGEAVRDGGQHARDPVGGSVEF
QFVPVLKLVSTLLVMGIFGDEDVKQILKMIEPEVFTEELEEGLLQMKLPE
SVKLQMCNLLEYFCDQELQHRVESLAAFAERYVDKLQANQRSRYALLMRA
FTMSAAETARRTREFRSPPQEQINMLLHFKDEADEEDCPLPEDIRQDLQD
FHQDLLAHCGIQLEKKPQSLQELVSHMVVRWAQEDYVQSPELVRAMFSLL
HRQYDGLGELLRALPRAYTISPSSVEDTMSLLECLGQIRSLLIVQMGPQE
ENLMIQSIGNIMNNKVFYQHPNLMRALGMHETVMEVMVNVLGGGETKEIR
FPKMVTSCCRFLCYFCRISRQNQRSMFDHLSYLLENSGIGLGMQGSTPLD
VAAASVIDNNELALALQEQDLEKVVSYLAGCGLQSCPMLLAKGYPDIGWN
PCGGERYLDFLRFAVFVNGESVEENANVVVRLLIRKPECFGPALRGEGGS
GLLAAIEEAIRISEDPARDGPGVPPEENRVHLGHAIMSFYAALIDLLGRC
APEMHLIQAGKGEALRIRAILRSLVPLDDLVGIISLPLQIPTLGKDGALV
QPKMSASFVPDHKASMVLFLDRVYGIENQDFLLHVLDVGFLPDMRAAASL
DTATFSTTEMALALNRYLCLAVLPLITKCAPLFAGTEHRAIMVDSMLHTV
YRLSRGRSLTKAQRDVIEDCLMALCRYIRPSMLQHLLRRLVFDVPILNEF
AKMPLKLLTNHYERCWKYYCLPTGWANFGVTSEEELHLTRKLFWGIFDSL
AHKKYDQELYRMAMPCLCAIAGALPPDYVDASYSSKAEKNFDPRPVETLN
VIIPEKLDSFINKFAEYTHEKWAFDKIQNNWSYGENVDEELKTHPMLRPY
KTFSEKDKEIYRWPIKESLKAMIAWEWTIEKAREGEYNPQPPDLSGVTLS
RELQAMAEQLAENYHNTWGRKKKQELEAKGGGTHPLLVPYDTLTAKEKAR
DREKAQELLKFLQMNGYAVTRLDTSSIEKRFAFGFLQQLLRWMDISQEFI
AHLEAVVSSGRVEKSPHEQEIKFFAKILLPLINQYFTNHCLYFLSTPAKV
LGSGGHASNKEKEMITSLFCKLAALVRHRVSLFGTDAPAVVNCLHILARS
LDARTVMKSGPEIVKAGLRSFFESASEDIEKMVENLRLGKVSKGVGQNLT
YTTVALLPVLTTLFQHIAQHQFGDDVILDDVQVSCYRTLCSIYSLGTTKN
TYVEKLRPALGECLARLAAAMPVAFLEPQLNEYNACSVYTTKSPRERAIL
GLPNSVEEMCPDIPVLDRLMADIGGLAESGARYTEMPHVIEITLPMLCSY
LPRWWERGPEAPPPALPAGAPPPCTAVTSDHLNSLLGNILRIIVNNLGID
EATWMKRLAVFAQPIVSRARPELLHSHFIPTIGRLRKRAGKVVAEEEQLR
LEAKAEAEEGELLVRDEFSVLCRDLYALYPLLIRYVDNNRAHWLTEPNAN
AEELFRMVGEIFIYWSKSHNFKREEQNFVVQNEINNMSFLTADSKSKMAR
RGDRYSVQTSLIVATLKKMLPIGLNMCAPTDQDLIMLAKTRYALKDTDEE
VREFLQNNLHLQGKVEGSPSLRWQMALYRGLPGREEDADDPEKIVRRVQE
VSAVLYHLEQTEHPYKSKKAVWHKLLSKQRRRAVVACFRMTPLYNLPTHR
ACNMFLESYKAAWILTEDHSFEDRMIDDLSKAGEQEEEEEEVEEKKPDPL
HQLVLHFSRTALTEKSKLDEDYLYMAYADIMAKSCHLEVEVSFEEKEMEK
QRLLYQQSRLHTRGAAEMVLQMISACKGETGAMVSSTLKLGISILNGGNA
EVQQKMLDYLKDKKEVGFFQSIQALMQTCSVLDLNAFERQNKAEGLGMVN
EDGTVINRQNGEKVMADDEFTQDLFRFLQLLCEGHNNDFQNYLRTQTGNT
TTINIIICTVDYLLRLQESISDFYWYYSGKDVIEEQGKRNFSKAMSVAKQ
VFNSLTEYIQGPCTGNQQSLAHSRLWDAVVGFLHVFAHMMMKLAQDSSQI
ELLKELLDLQKDMVVMLLSLLEGNVVNGMIARQMVDMLVESSSNVEMILK
FFDMFLKLKDIVGSEAFQDYVTDPRGLISKKDFQKAMDSQKQFTGPEIQF
LLSCSEADENEMINFEEFANRFQEPARDIGFNVAVLLTNLSEHVPHDPRL
RNFLELAESILEYFRPYLGRIEIMGASRRIERIYFEISETNRAQWEMPQV
KESKRQFIFDVVNEGGEAEKMELFVSFCEDTIFEMQIAAQISEPFWGELE
VQRVKFLNYLSRNFYTLRFLALFLAFAINFILLFYKVSDSPPNMVYYFLE
ESTGYMEPALWCLSLLHTLVAFLCIIGYNCLKVPLVIFKREKELARKLEF
DGLYITEQPGDDDVKGQWDRLVLNTPSFPSNYWDKFVKRKVLDKHGDIFG
RERIAELLGMDLASLEITAHNERKPDPPPGLLTWLMSIDVKYQIWKFGVI
FTDNSFLYLGWYMVMSLLGHYNNFFFAAHLLDIAMGVKTLRTILSSVTHN
GKQLVMTVGLLAVVVYLYTVVAFNFFRKFYNKSEDEDEPDMKCDDMMTCY
LFHMYVGVRAGGGIGDEIEDPAGDEYELYRVVFDITFFFFVIVILLAIIQ
GLIIDAFGELRDQQEQVKEDMETKCFICGIGSDYFDTTPHGFETHTLEEH
NLANYMFFLMYLINKDETEHTGQESYVWKMYQERCWDFFPAGDCFRKQYE
DQLS

3D structure

• PDB: 7tzc A drug and ATP binding site in type 1 ryanodine receptor.
• Chain: G
• Resolution: 2.45 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	L9R	G	W4794 L4801 Y4804 N4805 F4808	W4161 L4168 Y4171 N4172 F4175
BS02	ATP	G	K4214 R4215 M4954 E4955 C4958 T4979 H4983 L4985	K3904 R3905 M4321 E4322 C4325 T4346 H4350 L4352
BS03	CA	G	E3893 E3967 T5001	E3583 E3657 T4368
BS04	ZN	G	C4958 C4961 H4978 H4983	C4325 C4328 H4345 H4350
BS05	CFF	G	W4716 I4996	W4083 I4363
BS06	ATP	G	R897 H993 W996 R1020 N1035	R875 H971 W974 R998 N1013
BS07	KVR	G	H879 W882 C906 L913 E917 N921	H857 W860 C884 L891 E895 N899
BS08	L9R	G	Y4851 N4857 F4858 Y4909	Y4218 N4224 F4225 Y4276

Gene Ontology

Molecular Function

• GO:0005216 monoatomic ion channel activity
• GO:0005219 ryanodine-sensitive calcium-release channel activity
• GO:0005245 voltage-gated calcium channel activity
• GO:0005262 calcium channel activity
• GO:0005509 calcium ion binding
• GO:0005515 protein binding
• GO:0005516 calmodulin binding
• GO:0005524 ATP binding
• GO:0015278 intracellularly gated calcium channel activity
• GO:0015643 toxic substance binding
• GO:0035381 ATP-gated ion channel activity
• GO:0042802 identical protein binding
• GO:0044325 transmembrane transporter binding
• GO:0046872 metal ion binding
• GO:0097718 disordered domain specific binding

Biological Process

• GO:0003151 outflow tract morphogenesis
• GO:0006811 monoatomic ion transport
• GO:0006816 calcium ion transport
• GO:0006874 intracellular calcium ion homeostasis
• GO:0006936 muscle contraction
• GO:0006941 striated muscle contraction
• GO:0014808 release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
• GO:0019722 calcium-mediated signaling
• GO:0043588 skin development
• GO:0043931 ossification involved in bone maturation
• GO:0048741 skeletal muscle fiber development
• GO:0051209 release of sequestered calcium ion into cytosol
• GO:0051289 protein homotetramerization
• GO:0055085 transmembrane transport
• GO:0070588 calcium ion transmembrane transport
• GO:0071277 cellular response to calcium ion
• GO:0071313 cellular response to caffeine

Cellular Component

• GO:0005790 smooth endoplasmic reticulum
• GO:0014802 terminal cisterna
• GO:0016020 membrane
• GO:0016529 sarcoplasmic reticulum
• GO:0030018 Z disc
• GO:0031090 organelle membrane
• GO:0033017 sarcoplasmic reticulum membrane
• GO:0034704 calcium channel complex
• GO:0042383 sarcolemma
• GO:1990425 ryanodine receptor complex

External links

• PDB: RCSB:7tzc, PDBe:7tzc, PDBj:7tzc
• PDBsum: 7tzc
• PubMed: 35580609
• UniProt: P11716|RYR1_RABIT Ryanodine receptor 1 (Gene Name=RYR1)