Structure of PDB 7r48 Chain G

Receptor sequence

>7r48G (length=688) Species: 9913 (Bos taurus)

SNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMC
LVEIEKAPKVVAACAMPVMKGWNILTNSEKTKKAREGVMEFLLANHPLDC
PICDQGGECDLQDQSMMFGSDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQ
CTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNIIDICPV
GALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMH
EDINEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTHTTWEDALSRVAG
MLQSFQGNDVAAIAGGLVDAEALIALKDLLNRVDSDTLCTEEVFPTAGAG
TDLRSNYLLNTTIAGVEEADVVLLVGTNPRFEAPLFNARIRKSWLHNDLK
VALIGSPVDLTYRYDHLGDSPKILQDIASGSHPFSQVLQEAKKPMVILGS
SALQRNDGAAILAAVSNIAQKIRTSSGVTGDWKVMNILHRIASQVAALDL
GYKPGVEAIQKNPPKMLFLLGADGGCITRQDLPKDCFIVYQGHHGDVGAP
IADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLAREDWKIIRALSE
IAGMTLPYDTLDQVRNRLEEVSPNLVRYDDVEGANYFQQASELSKLVNQQ
LLADPLVPPQLTIKDFYMTDSISRASQTMAKCVKAVTE

3D structure

• PDB: 7r48 Structural basis of mammalian respiratory complex I inhibition by medicinal biguanides.
• Chain: G
• Resolution: 2.3 Å

Enzymatic activity

• Enzyme Commision number: 7.1.1.2: NADH:ubiquinone reductase (H(+)-translocating).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	G	H101 D104 C105 C108 Q110 G111 C114 Q117 V205	H96 D99 C100 C103 Q105 G106 C109 Q112 V200
BS02	SF4	G	C153 I154 Q155 C156 T157 R158 C159 C203 P204 A207 L208	C148 I149 Q150 C151 T152 R153 C154 C198 P199 A202 L203
BS03	FES	G	R39 C41 Y42 G50 C52 R53 C55 C69	R34 C36 Y37 G45 C47 R48 C50 C64

Gene Ontology

Molecular Function

• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0009055 electron transfer activity
• GO:0016491 oxidoreductase activity
• GO:0016651 oxidoreductase activity, acting on NAD(P)H
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051537 2 iron, 2 sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0006120 mitochondrial electron transport, NADH to ubiquinone
• GO:0032981 mitochondrial respiratory chain complex I assembly
• GO:0042773 ATP synthesis coupled electron transport
• GO:0045333 cellular respiration
• GO:1902600 proton transmembrane transport

Cellular Component

• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane
• GO:0005758 mitochondrial intermembrane space
• GO:0016020 membrane
• GO:0045271 respiratory chain complex I

External links

• PDB: RCSB:7r48, PDBe:7r48, PDBj:7r48
• PDBsum: 7r48
• PubMed: 36701435
• UniProt: P15690|NDUS1_BOVIN NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (Gene Name=NDUFS1)