Structure of PDB 7qso Chain G

Receptor sequence

>7qsoG (length=700) Species: 9913 (Bos taurus)

ASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRM
CLVEIEKAPKVVAACAMPVMKGWNILTNSEKTKKAREGVMEFLLANHPLD
CPICDQGGECDLQDQSMMFGSDRSRFLEGKRAVEDKNIGPLVKTIMTRCI
QCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNIIDICP
VGALTSKPYAFTARPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRM
HEDINEEWISDKTRFAYDGLKRQRLTEPMVRNEKGLLTHTTWEDALSRVA
GMLQSFQGNDVAAIAGGLVDAEALIALKDLLNRVDSDTLCTEEVFPTAGA
GTDLRSNYLLNTTIAGVEEADVVLLVGTNPRFEAPLFNARIRKSWLHNDL
KVALIGSPVDLTYRYDHLGDSPKILQDIASGSHPFSQVLQEAKKPMVILG
SSALQRNDGAAILAAVSNIAQKIRTSSGVTGDWKVMNILHRIASQVAALD
LGYKPGVEAIQKNPPKMLFLLGADGGCITRQDLPKDCFIVYQGHHGDVGA
PIADVILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGLAREDWKIIRALS
EIAGMTLPYDTLDQVRNRLEEVSPNLVRYDDVEGANYFQQASELSKLVNQ
QLLADPLVPPQLTIKDFYMTDSISRASQTMAKCVKAVTEGAHAVEEPSIC

3D structure

• PDB: 7qso Cryo-EM structures define ubiquinone-10 binding to mitochondrial complex I and conformational transitions accompanying Q-site occupancy.
• Chain: G
• Resolution: 3.02 Å

Enzymatic activity

• Enzyme Commision number: 7.1.1.2: NADH:ubiquinone reductase (H(+)-translocating).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	G	H101 D104 C105 C108 Q110 G111 C114 Q117 V205 G206	H97 D100 C101 C104 Q106 G107 C110 Q113 V201 G202
BS02	SF4	G	C153 I154 Q155 C156 T157 R158 C159 C203 P204 A207	C149 I150 Q151 C152 T153 R154 C155 C199 P200 A203
BS03	FES	G	R39 C41 Y42 G50 C52 R53 C55 C69	R35 C37 Y38 G46 C48 R49 C51 C65

Gene Ontology

Molecular Function

• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0009055 electron transfer activity
• GO:0016491 oxidoreductase activity
• GO:0016651 oxidoreductase activity, acting on NAD(P)H
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051537 2 iron, 2 sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0006120 mitochondrial electron transport, NADH to ubiquinone
• GO:0032981 mitochondrial respiratory chain complex I assembly
• GO:0042773 ATP synthesis coupled electron transport
• GO:0045333 cellular respiration
• GO:1902600 proton transmembrane transport

Cellular Component

• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane
• GO:0005758 mitochondrial intermembrane space
• GO:0016020 membrane
• GO:0045271 respiratory chain complex I

External links

• PDB: RCSB:7qso, PDBe:7qso, PDBj:7qso
• PDBsum: 7qso
• PubMed: 35589726
• UniProt: P15690|NDUS1_BOVIN NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (Gene Name=NDUFS1)