Structure of PDB 7ohq Chain G

Receptor sequence

>7ohqG (length=233) Species: 559292 (Saccharomyces cerevisiae S288C)

NPLTHSTPKNFGIGQAVQPKRNLSRYVKWPEYVRVQRQKKILSIRLKVPP
TIAQFQYTLDRNTAAETFKLFNKYRPETAAEKKERLTKEAAAVAEGKSKQ
DASPKPYAVKYGLNHVVALIENKKAKLVLIANDVDPIELVVFLPALCKKM
GVPYAIVKGKARLGTLVNQKTSAVAALTEVRAEDEAALAKLVSTIDANFA
DKYDEVKKHWGGGILGNKAQAKMDKRAKNSDSA

3D structure

• PDB: 7ohq Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
• Chain: G
• Resolution: 3.1 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	G	H28 S29 T30 P31 K32 N33 F34 G35 I36 G37 Q41 P42 K43 L46 R48 Y49 K51 P53 E54 Y55 R57 K63 I64 L65 I67 L69 K70 K105 K106 R108 Q123 S126 K128 P129 K133 Y134 G135 L136 N137 H138 A141 N145 P159 L162 K183 A184 Q192 K193 T194 S195 N240 K241 A242 A256	H5 S6 T7 P8 K9 N10 F11 G12 I13 G14 Q18 P19 K20 L23 R25 Y26 K28 P30 E31 Y32 R34 K40 I41 L42 I44 L46 K47 K82 K83 R85 Q100 S103 K105 P106 K110 Y111 G112 L113 N114 H115 A118 N122 P136 L139 K160 A161 Q169 K170 T171 S172 N217 K218 A219 A233
BS02	rna	G	W52 Y55 V56 Q59 R60 R84 K181 R185	W29 Y32 V33 Q36 R37 R61 K158 R162
BS03	rna	G	E89 A212 S216	E66 A189 S193

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000470 maturation of LSU-rRNA
• GO:0002181 cytoplasmic translation
• GO:0006364 rRNA processing
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ohq, PDBe:7ohq, PDBj:7ohq
• PDBsum: 7ohq
• PubMed: 34813592
• UniProt: P17076|RL8A_YEAST Large ribosomal subunit protein eL8A (Gene Name=RPL8A)