Structure of PDB 7nyu Chain G

Receptor sequence

>7nyuG (length=907) Species: 37762 (Escherichia coli B)

MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGACRQCAV
KQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHP
HDCPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNR
CIACYRCVRYYKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEI
CPTGVFTDKTHSERYNRKWDMQFAPSICQQCSIGCNISPGERYGELRRIE
NRYNGTVNHYFLCDRGRFGYGYVNLKDRPRQPVQRRGDDFITLNAEQAMQ
GAADILRQSKKVIGIGSPRASVESNFALRELVGEENFYTGIAHGEQERLQ
LALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALAVRQAVKGK
AREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIIS
GTNAGSLEVIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLE
EALTELETGRADAVVVLENDLHRHASAIRVNAALAKAPLVMVVDHQRTAI
MENAHLVLSAASFAESDGTVINNEGRAQRFFQVYDPAYYDSKTVMLESWR
WLHSLHSTLLSREVDWTQLDHVIDAVVAKIPELAGIKDAAPDATFRIRGQ
KLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEGNNQPTAHRSQ
VPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTSVP
ARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKL
GVNAGTRVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHL
EDLKEAQ

3D structure

• PDB: 7nyu Structure of Escherichia coli respiratory complex I reconstituted into lipid nanodiscs reveals an uncoupled conformation.
• Chain: G
• Resolution: 3.8 Å

Enzymatic activity

• Enzyme Commision number: 7.1.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FES	G	F33 C34 G43 A44 C45 R46 C48 C67	F33 C34 G43 A44 C45 R46 C48 C67
BS02	SF4	G	C151 I152 A153 C154 Y155 R156 C157 F181 C201 P202 V205	C151 I152 A153 C154 Y155 R156 C157 F181 C201 P202 V205
BS03	SF4	G	H99 D102 C103 C106 E108 C112 Q115 R150 T203	H99 D102 C103 C106 E108 C112 Q115 R150 T203
BS04	SF4	G	C228 Q230 C231 I233 G234 C235 C263 G266	C228 Q230 C231 I233 G234 C235 C263 G266
BS05	CA	G	D617 Q632 E647 D731	D617 Q632 E647 D731

Gene Ontology

Molecular Function

• GO:0003954 NADH dehydrogenase activity
• GO:0005515 protein binding
• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0010181 FMN binding
• GO:0016491 oxidoreductase activity
• GO:0016651 oxidoreductase activity, acting on NAD(P)H
• GO:0043546 molybdopterin cofactor binding
• GO:0046872 metal ion binding
• GO:0048038 quinone binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051537 2 iron, 2 sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0009060 aerobic respiration
• GO:0022904 respiratory electron transport chain
• GO:0042773 ATP synthesis coupled electron transport
• GO:0045333 cellular respiration
• GO:1902600 proton transmembrane transport

Cellular Component

• GO:0005737 cytoplasm
• GO:0005886 plasma membrane
• GO:0016020 membrane
• GO:0030964 NADH dehydrogenase complex
• GO:0045271 respiratory chain complex I
• GO:1990204 oxidoreductase complex

External links

• PDB: RCSB:7nyu, PDBe:7nyu, PDBj:7nyu
• PDBsum: 7nyu
• PubMed: 34308841
• UniProt: P33602|NUOG_ECOLI NADH-quinone oxidoreductase subunit G (Gene Name=nuoG)