Structure of PDB 7miu Chain G

Receptor sequence

>7miuG (length=542) Species: 10090 (Mus musculus)

MKYILVTGGVISGIGKGIIASSIGTILKSCGLRVTAIKIDPYINIDAGTF
SPYEHGEVFVLNDGGEVDLDLGNYERFLDINLYKDNNITTGKIYQHVINK
ERRGDYLGKTVQVVPHITDAIQEWVMNQAKVSVDGNKEDPQICVIELGGT
IGDIEGMAFVEAFRQFQFKAKKENFYNIHVSLVPQPSATGEQKTKPTQNS
VRALRGLGLSPDLIVCRSSTPIEMAVKEKISMFCHVNPEQVICIHDVSSI
YRVPLLLEEQGVVKYFQERLGLPLLFKWKAMADRYERLQKICSIALVGKY
TKLRDCYASVFKALEHSALAINHKLNLMYIDSIDLEPVTKAEDPVKFHEA
WQKLCLADGILVPGGFGIRGTLGKLQAISWARTKKIPFLGICLGMQLAVI
EFARNCLNLKDANSTEFEPNTPVPLVIDMPETMRLGLRRTVFTTENSILK
KLYGDVPYIEERHRHRYEVNPNLINQFENKDLCFVGEDVDGKRMEIVELT
SHPYFIGVQFHPEFSSRPMKPSPPYLGLLLAATGNLNAHLQQ

3D structure

• PDB: 7miu Structural basis for isoform-specific inhibition of human CTPS1.
• Chain: G
• Resolution: 2.6 Å

Enzymatic activity

• Enzyme Commision number: 6.3.4.2: CTP synthase (glutamine hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	UTP	G	K38 D40 H55 G148 G149 D153	K38 D40 H55 G148 G149 D153
BS02	GLN	G	G371 G372 F373 C399 L400 E423 H480 R481 Y482 H526	G364 G365 F366 C392 L393 E416 H465 R466 Y467 H511
BS03	UTP	G	E191 K193 T194 K195 Q198 K229	E191 K193 T194 K195 Q198 K229
BS04	ZG4	G	I19 R76 F77 R217 D246 V247 I250 Y251 V253 W285 F318 K319 E322 H323	I19 R76 F77 R217 D246 V247 I250 Y251 V253 W278 F311 K312 E315 H316

Gene Ontology

Molecular Function

• GO:0003883 CTP synthase activity
• GO:0005524 ATP binding
• GO:0016874 ligase activity
• GO:0016879 ligase activity, forming carbon-nitrogen bonds
• GO:0042802 identical protein binding

Biological Process

• GO:0006221 pyrimidine nucleotide biosynthetic process
• GO:0006241 CTP biosynthetic process
• GO:0006541 glutamine metabolic process
• GO:0044210 'de novo' CTP biosynthetic process

Cellular Component

• GO:0005739 mitochondrion

External links

• PDB: RCSB:7miu, PDBe:7miu, PDBj:7miu
• PDBsum: 7miu
• PubMed: 34583994
• UniProt: P70303|PYRG2_MOUSE CTP synthase 2 (Gene Name=Ctps2)