Structure of PDB 7fs0 Chain G

Receptor sequence

>7fs0G (length=421) Species: 9606 (Homo sapiens)

AFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVER
LKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAI
ALDTKGPGPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGP
EGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQ
KMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIM
LSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTE
VTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAA
RQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVI
VVTGWRPGSGYTNIMRVLSIS

3D structure

• PDB: 7fs0 Tuning liver pyruvate kinase activity up or down with a new class of allosteric modulators.
• Chain: G
• Resolution: 2.412 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.40: pyruvate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FBP	G	L443 T444 T445 T446 S449 R501 G526 R528 P529 G530 S531 G532 Y533 T534	L321 T322 T323 T324 S327 R379 G404 R406 P407 G408 S409 G410 Y411 T412
BS02	OXL	G	K282 E284 A305 G307 D308 T340	K160 E162 A183 G185 D186 T218
BS03	MG	G	E284 D308	E162 D186
BS04	O8X	G	F38 L39 Y402 Q405 L406 E409	F16 L17 Y280 Q283 L284 E287

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0004743 pyruvate kinase activity
• GO:0030955 potassium ion binding

Biological Process

• GO:0006096 glycolytic process

External links

• PDB: RCSB:7fs0, PDBe:7fs0, PDBj:7fs0
• PDBsum: 7fs0
• PubMed: 36753880
• UniProt: P30613|KPYR_HUMAN Pyruvate kinase PKLR (Gene Name=PKLR)