Structure of PDB 7awt Chain G

Receptor sequence

>7awtG (length=907) Species: 562 (Escherichia coli)

ATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGACRQCAVK
QYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPH
DCPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRC
IACYRCVRYYKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEIC
PTGVFTDKTHSERYNRKWDMQFAPSICQQCSIGCNISPGERYGELRRIEN
RYNGTVNHYFLCDRGRFGYGYVNLKDRPRQPVQRRGDDFITLNAEQAMQG
AADILRQSKKVIGIGSPRASVESNFALRELVGEENFYTGIAHGEQERLQL
ALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALAVRQAVKGKA
REMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAPV
EDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISG
TNAGSLEVIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEE
ALTELETGRADAVVVLENDLHRHASAIRVNAALAKAPLVMVVDHQRTAIM
ENAHLVLSAASFAESDGTVINNEGRAQRFFQVYDPAYYDSKTVMLESWRW
LHSLHSTLLSREVDWTQLDHVIDAVVAKIPELAGIKDAAPDATFRIRGQK
LAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEGNNQPTAHRSQV
PFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTSVPA
RFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLG
VNAGTRVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLE
DLKEAQQ

3D structure

• PDB: 7awt Structure of the peripheral arm of a minimalistic respiratory complex I.
• Chain: G
• Resolution: 2.73 Å

Enzymatic activity

• Enzyme Commision number: 7.1.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FES	G	Y32 C34 W35 G43 C45 R46 C48 C67	Y31 C33 W34 G42 C44 R45 C47 C66
BS02	SF4	G	C151 I152 A153 C154 Y155 C157 F181 C201 P202 T203 V205	C150 I151 A152 C153 Y154 C156 F180 C200 P201 T202 V204
BS03	SF4	G	H99 D102 C103 C106 C112 Q115 T203 G204	H98 D101 C102 C105 C111 Q114 T202 G203
BS04	SF4	G	C228 Q230 C231 I233 C235 C263 R388	C227 Q229 C230 I232 C234 C262 R387
BS05	MG	G	D617 Q632 E647 D731	D616 Q631 E646 D730
BS06	MG	G	V198 C201 T203	V197 C200 T202

Gene Ontology

Molecular Function

• GO:0003954 NADH dehydrogenase activity
• GO:0005515 protein binding
• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0010181 FMN binding
• GO:0016491 oxidoreductase activity
• GO:0016651 oxidoreductase activity, acting on NAD(P)H
• GO:0043546 molybdopterin cofactor binding
• GO:0046872 metal ion binding
• GO:0048038 quinone binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051537 2 iron, 2 sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0009060 aerobic respiration
• GO:0022904 respiratory electron transport chain
• GO:0042773 ATP synthesis coupled electron transport
• GO:0045333 cellular respiration
• GO:1902600 proton transmembrane transport

Cellular Component

• GO:0005737 cytoplasm
• GO:0005886 plasma membrane
• GO:0016020 membrane
• GO:0030964 NADH dehydrogenase complex
• GO:0045271 respiratory chain complex I
• GO:1990204 oxidoreductase complex

External links

• PDB: RCSB:7awt, PDBe:7awt, PDBj:7awt
• PDBsum: 7awt
• PubMed: 34562374
• UniProt: P33602|NUOG_ECOLI NADH-quinone oxidoreductase subunit G (Gene Name=nuoG)