Structure of PDB 6oig Chain G

Receptor sequence

>6oigG (length=233) Species: 4932 (Saccharomyces cerevisiae)

NPLTHSTPKNFGIGQAVQPKRNLSRYVKWPEYVRVQRQKKILSIRLKVPP
TIAQFQYTLDRNTAAETFKLFNKYRPETAAEKKERLTKEAAAVAEGKSKQ
DASPKPYAVKYGLNHVVALIENKKAKLVLIANDVDPIELVVFLPALCKKM
GVPYAIVKGKARLGTLVNQKTSAVAALTEVRAEDEAALAKLVSTIDANFA
DKYDEVKKHWGGGILGNKAQAKMDKRAKNSDSA

3D structure

• PDB: 6oig An open interface in the pre-80S ribosome coordinated by ribosome assembly factors Tsr1 and Dim1 enables temporal regulation of Fap7.
• Chain: G
• Resolution: 3.8 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	G	T191 H192 S193 T194 K196 N197 F198 G199 I200 G201 Q202 P206 K207 R208 L210 S211 R212 Y213 V214 K215 E218 Y219 R224 L229 R232 L233 K269 R272 Q287 D288 G299 L300 H302 A305 N309 D322 P323 K347 A348 K357 L402 G403 N404 K405 A406 K412	T4 H5 S6 T7 K9 N10 F11 G12 I13 G14 Q15 P19 K20 R21 L23 S24 R25 Y26 V27 K28 E31 Y32 R37 L42 R45 L46 K82 R85 Q100 D101 G112 L113 H115 A118 N122 D135 P136 K160 A161 K170 L215 G216 N217 K218 A219 K225
BS02	rna	G	W216 Y219 Q223 R224 K227 R248 K345 R349	W29 Y32 Q36 R37 K40 R61 K158 R162

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000470 maturation of LSU-rRNA
• GO:0002181 cytoplasmic translation
• GO:0006364 rRNA processing
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6oig, PDBe:6oig, PDBj:6oig
• PDBsum: 6oig
• PubMed: 33219089
• UniProt: P17076|RL8A_YEAST Large ribosomal subunit protein eL8A (Gene Name=RPL8A)