Structure of PDB 5yei Chain G

Receptor sequence

>5yeiG (length=397) Species: 208964 (Pseudomonas aeruginosa PAO1)

ALIVQKFGGTSVGTVERIEQVAEKVKKFREAGDDVVVVVSAMSGETNRLI
GLANQIMEQPVPRELDVMVSTGEQVTIALLSMALIKRGVPAVSYTGNQVR
ILTDSAHTKARILHIDDTHIRADLKAGRVVVVAGFQGVDGNGNITTLGRG
GSDTTGVALAAALKADECQIYTDVDGVYTTDPRVVPQARRLDKITFEEML
EMASLGSKVLQIRAVEFAGKYNVPLRVLHSFQEGPGTLITIDPIISGIAF
NRDEAKLTIRGVPDTPGVAFKILGPISAANVEVDMIVQNVAHDNTTDFTF
TVHRNDYLNALEILKQTAANIGAREAIGDTNIAKVSIVGVGMRSHAGVAS
RMFEALAKESINIQMISTSEIKVSVVIEEKYLELAVRALHTAFELDA

3D structure

• PDB: 5yei Mechanistic insights into the allosteric regulation of Pseudomonas aeruginosa aspartate kinase.
• Chain: G
• Resolution: 2.301 Å

Enzymatic activity

• Enzyme Commision number: 2.7.2.4: aspartate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	THR	G	N371 I372	N362 I363
BS02	LYS	G	M351 H354 G356 V357 A358 S378 V382	M342 H345 G347 V348 A349 S369 V373
BS03	THR	G	T274 P275 G276 V277 A278 Q297	T265 P266 G267 V268 A269 Q288

Gene Ontology

Molecular Function

• GO:0004072 aspartate kinase activity
• GO:0005524 ATP binding
• GO:0016301 kinase activity

Biological Process

• GO:0008652 amino acid biosynthetic process
• GO:0009085 lysine biosynthetic process
• GO:0009088 threonine biosynthetic process
• GO:0009089 lysine biosynthetic process via diaminopimelate
• GO:0009090 homoserine biosynthetic process
• GO:0016310 phosphorylation
• GO:0019877 diaminopimelate biosynthetic process

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:5yei, PDBe:5yei, PDBj:5yei
• PDBsum: 5yei
• PubMed: 29382741
• UniProt: O69077|AK_PSEAE Aspartokinase (Gene Name=lysC)