Structure of PDB 5utu Chain G

Receptor sequence

>5utuG (length=495) Species: 353152 (Cryptosporidium parvum Iowa II)

YKMESRIKDISLAEFGLQDMEIAKTDMMGLVELQRKYRDSKPLKGARITG
SLHLTIETSVLVETLYELGAEIRWCSCNIYSTQDHAAAALVKKNIATVFA
WKNETIEDYWVCLNDAMTWRNPNDKDKICGPNLIVDDGGDATLILHEGVK
AEIEYEKYNKIPEYLETELDENGKQLSMDLKCMYKVLKMELLKNPFRWRG
MLKDLYGVSEETTTGVLRLKIMESEGKLLLPAINVNDSVTKSKFDNTYGC
RQSLLHGLFNGCIQMLAGKKIVVLGYGEVGKGCAQGLSGVGARVIVTEID
PICALQASMEGYQVSVLEDVVSEADIFITATGNKDVITVEHMRKMKENAY
IANIGHFDDEIDVYGLENYPGIKVIEVKQNVHKFTFPDTQKSVILLCKGR
LVNLGCATGHPPLVMSMSFTNQVLAQMDLWKSRELVDRSKNTRFFVKKLS
KELDEYVARLHLDVLGIKLTKLTETQAKYINVSINGPYKSEDYRY

3D structure

• PDB: 5utu 2.65 Angstrom Resolution Crystal Structure of S-adenosylhomocysteinase from Cryptosporidium parvum in Complex with SAH and NAD
• Chain: G
• Resolution: 2.65 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H53 S76 S81 D137 E211 N236 K241 D245 N246 C250 H356 H410 S418 Q422
• Catalytic site (residue number reindexed from 1): H53 S76 S81 D137 E211 N236 K241 D245 N246 C250 H356 H410 S418 Q422
• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	G	N246 C250 G277 E278 V279 E298 I299 T331 N333 I354 G355 N403 H410	N246 C250 G277 E278 V279 E298 I299 T331 N333 I354 G355 N403 H410
BS02	SAH	G	L52 H53 T55 E57 C77 N78 D137 E211 T212 D245 H356 F357 L401 H410 M415	L52 H53 T55 E57 C77 N78 D137 E211 T212 D245 H356 F357 L401 H410 M415
BS03	NAD	G	K489 Y493	K489 Y493

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:5utu, PDBe:5utu, PDBj:5utu
• PDBsum: 5utu
• UniProt: Q5CPH1