Structure of PDB 5scl Chain G

Receptor sequence

>5sclG (length=423) Species: 9606 (Homo sapiens)

GTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSV
ERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPV
AIALDTKGPGPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAAL
GPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFL
AQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADC
IMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDP
TEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQ
AARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDL
VIVVTGWRPGSGYTNIMRVLSIS

3D structure

• PDB: 5scl Anthraquinone derivatives as ADP-competitive inhibitors of liver pyruvate kinase.
• Chain: G
• Resolution: 2.134 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.40: pyruvate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FBP	G	L443 T444 T445 T446 S449 W494 R501 G526 R528 P529 G530 S531 G532 Y533 T534	L323 T324 T325 T326 S329 W374 R381 G406 R408 P409 G410 S411 G412 Y413 T414
BS02	OXL	G	K282 E284 A305 G307 D308 T340	K162 E164 A185 G187 D188 T220
BS03	AZN	G	P65 N87 H90 G91 Y95 S374 G375 K379	P45 N67 H70 G71 Y75 S254 G255 K259
BS04	MG	G	E284 D308	E164 D188

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0004743 pyruvate kinase activity
• GO:0030955 potassium ion binding

Biological Process

• GO:0006096 glycolytic process

External links

• PDB: RCSB:5scl, PDBe:5scl, PDBj:5scl
• PDBsum: 5scl
• PubMed: 35290845
• UniProt: P30613|KPYR_HUMAN Pyruvate kinase PKLR (Gene Name=PKLR)