Structure of PDB 5scf Chain G

Receptor sequence

>5scfG (length=422) Species: 9606 (Homo sapiens)

GTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSV
ERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPV
AIALDTKGPPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALG
PEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLA
QKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCI
MLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPT
EVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQA
ARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLV
IVVTGWRPGSGYTNIMRVLSIS

3D structure

• PDB: 5scf Anthraquinone derivatives as ADP-competitive inhibitors of liver pyruvate kinase.
• Chain: G
• Resolution: 2.185 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.40: pyruvate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FBP	G	T444 T445 T446 S449 W494 R501 G526 R528 P529 G530 S531 G532 Y533 T534	T323 T324 T325 S328 W373 R380 G405 R407 P408 G409 S410 G411 Y412 T413
BS02	OXL	G	K282 E284 A305 G307 D308 T340	K161 E163 A184 G186 D187 T219
BS03	I91	G	P65 N87 H90 G91 Y95 S374 G375 A378 K379	P45 N67 H70 G71 Y75 S253 G254 A257 K258
BS04	MG	G	E284 D308	E163 D187

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0004743 pyruvate kinase activity
• GO:0030955 potassium ion binding

Biological Process

• GO:0006096 glycolytic process

External links

• PDB: RCSB:5scf, PDBe:5scf, PDBj:5scf
• PDBsum: 5scf
• PubMed: 35290845
• UniProt: P30613|KPYR_HUMAN Pyruvate kinase PKLR (Gene Name=PKLR)