Structure of PDB 5ntd Chain G

Receptor sequence

>5ntdG (length=520) Species: 5702 (Trypanosoma brucei brucei)

PTLPKAEAKELSAFVQSCVEYKTNVCFTDVAAYESNQKGVLSSGLAVLVG
THKQLRDPAVQRLPFYNPAVAEAIERVKEGGTYGVLVEGLANAAGSKFVR
VVVGEVPTKASRNNCPARPDVVTALVTAALDEVKEPNTTVDVFVLSNAVL
PIAAAVARCGKHNFSAKDGAAAAAYNSGKVSRLQVVFPEPPAIPPKDLEA
VATSTQLCQRLVDAPPNLLTTATFTEIAQGYAKALGFDVDVICGDDLCER
GYGGIYSVGKAAFEAPRLVTLLYTPKGTPVKKVSLVGKGIVYDCGGLALK
PADYMKLMKHDMGGAAAVFCGFLTAVRLQQPVQLSCTLCLAENAIGPKSY
RNDDIIVMKSGKTVEVINTDAEGRIVLGDGVFHATNELSFTPDVVIDMAT
LTGAQGIATGRHHAGLYVNEEGAEAAMLRAGRESGETCFPVLYCPEYHEP
EFKSNHADMTNLMERRDNAGVSCAGYFITTHLSPKFTGAHIHVDLAYPVF
NSNGATGFGPALLTEYFRKL

3D structure

• PDB: 5ntd Structural Characterization of Acidic M17 Leucine Aminopeptidases from the TriTryps and Evaluation of Their Role in Nutrient Starvation inTrypanosoma brucei.
• Chain: G
• Resolution: 2.3 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K301 R375
• Catalytic site (residue number reindexed from 1): K300 R374

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MN	G	D294 K301 D371 E373	D293 K300 D370 E372
BS02	MN	G	K289 D294 D312 E373	K288 D293 D311 E372
BS03	BES	G	K289 D294 K301 M309 D312 D371 E373 T401 L402 G404 R467	K288 D293 K300 M308 D311 D370 E372 T400 L401 G403 R466
BS04	BCT	G	A372 G374 R375 L402	A371 G373 R374 L401

Gene Ontology

Molecular Function

• GO:0004177 aminopeptidase activity
• GO:0005515 protein binding
• GO:0008233 peptidase activity
• GO:0030145 manganese ion binding
• GO:0046872 metal ion binding
• GO:0070006 metalloaminopeptidase activity

Biological Process

• GO:0006508 proteolysis
• GO:0019538 protein metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:5ntd, PDBe:5ntd, PDBj:5ntd
• PDBsum: 5ntd
• PubMed: 28815215
• UniProt: Q385B0