Structure of PDB 5nrl Chain G

Receptor sequence

>5nrlG (length=372) Species: 4932 (Saccharomyces cerevisiae)

EKFQGHGLKTELHSALKSSNLNLIRRTYQTGENPYLSNRRYERGLKFKRI
AQERTLQKQQEEEELKRKLKQEEDEKDKRKLIESGDPNLELHEDKFLLDL
SKFKIYYDNNHGYEWWDTAYHPSIRYVAHPLPEKAKVSIKAYLTQHERKR
LRRNRRKMAREAREIKIKLGLLPKPEPKVKLSNMMSVFENDQNITDPTAW
EKVVKDQVDLRKRKHLEENERRHEDAIKRRKEAVNMNVEKPTVYHCKVFQ
FKNLQNPKIRFKLKMNSKELSLKGLCLRIRDDGPGIIIVVGNEKSCKFYE
NLVMKRIKWNEDFELHTGDIKMDMHNNSISKTWEGYLQDCKFKGWFMKVC
NDQDSLLRTLGQFDSEHFYSPV

3D structure

• PDB: 5nrl Structure of a pre-catalytic spliceosome.
• Chain: G
• Resolution: 7.2 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	G	H239 R241 R245 R246 R249 E257 K267 K271 K273 E282 H308	H146 R148 R152 R153 R156 E164 K174 K178 K180 E189 H215
BS02	rna	G	R253 K271 N276 S279 E282 N312 H316 A319 R323 P350 K351 R353 F354 K357 M358 E362 R371 K387 N394 R399 F441 M442 K443 Q457	R160 K178 N183 S186 E189 N219 H223 A226 R230 P257 K258 R260 F261 K264 M265 E269 R278 K294 N301 R306 F346 M347 K348 Q362

Gene Ontology

Molecular Function

• GO:0003674 molecular_function
• GO:0005515 protein binding

Biological Process

• GO:0000245 spliceosomal complex assembly
• GO:0000387 spliceosomal snRNP assembly
• GO:0000398 mRNA splicing, via spliceosome
• GO:0006397 mRNA processing
• GO:0008380 RNA splicing

Cellular Component

• GO:0005634 nucleus
• GO:0005681 spliceosomal complex
• GO:0005687 U4 snRNP
• GO:0046540 U4/U6 x U5 tri-snRNP complex
• GO:0071001 U4/U6 snRNP

External links

• PDB: RCSB:5nrl, PDBe:5nrl, PDBj:5nrl
• PDBsum: 5nrl
• PubMed: 28530653
• UniProt: Q03338|PRP3_YEAST U4/U6 small nuclear ribonucleoprotein PRP3 (Gene Name=PRP3)