Structure of PDB 5lj5 Chain G

Receptor sequence
>5lj5G (length=97) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]
MSRNVDKANSVLVRFQEQQAESAGGYKDYSRYQRPRNVSKVKSIKEANEW
KRQVSKEIKQKSTRIYDPSLNEMQIAELNDELNNLFKEWKRWQWHID
3D structure
PDB5lj5 Cryo-EM structure of the spliceosome immediately after branching.
ChainG
Resolution10.0 Å
3D
structure
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Enzymatic activity
Enzyme Commision number ?
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna G S2 R3 K40 R52 K59 Q60 S2 R3 K40 R52 K59 Q60
BS02 rna G M1 S10 V11 R14 M1 S10 V11 R14
BS03 rna G N4 S30 R31 Q60 N4 S30 R31 Q60
Gene Ontology
Molecular Function
GO:0000384 first spliceosomal transesterification activity
GO:0005515 protein binding
Biological Process
GO:0000350 generation of catalytic spliceosome for second transesterification step
GO:0000389 mRNA 3'-splice site recognition
GO:0006397 mRNA processing
GO:0008380 RNA splicing
Cellular Component
GO:0000974 Prp19 complex
GO:0005634 nucleus
GO:0005681 spliceosomal complex
GO:0005737 cytoplasm
GO:0071006 U2-type catalytic step 1 spliceosome
GO:0071007 U2-type catalytic step 2 spliceosome
GO:0071013 catalytic step 2 spliceosome
GO:0071014 post-mRNA release spliceosomal complex
GO:0071020 post-spliceosomal complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5lj5, PDBe:5lj5, PDBj:5lj5
PDBsum5lj5
PubMed27459055
UniProtP21374|ISY1_YEAST Pre-mRNA-splicing factor ISY1 (Gene Name=ISY1)

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