Structure of PDB 5h4p Chain G

Receptor sequence

>5h4pG (length=233) Species: 559292 (Saccharomyces cerevisiae S288C)

NPLTHSTPKNFGIGQAVQPKRNLSRYVKWPEYVRVQRQKKILSIRLKVPP
TIAQFQYTLDRNTAAETFKLFNKYRPETAAEKKERLTKEAAAVAEGKSKQ
DASPKPYAVKYGLNHVVALIENKKAKLVLIANDVDPIELVVFLPALCKKM
GVPYAIVKGKARLGTLVNQKTSAVAALTEVRAEDEAALAKLVSTIDANFA
DKYDEVKKHWGGGILGNKAQAKMDKRAKNSDSA

3D structure

• PDB: 5h4p Structural snapshot of cytoplasmic pre-60S ribosomal particles bound by Nmd3, Lsg1, Tif6 and Reh1
• Chain: G
• Resolution: 3.07 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	G	T27 H28 T30 P31 K32 N33 F34 G35 G37 Q38 Q41 P42 R44 L46 S47 R48 Y49 K51 E54 Y55 R57 R60 R68 L69 E100 K105 R108 D124 S126 P127 K128 P129 K133 Y134 G135 L136 H138 L142 K147 P159 L162 K183 A184 Q192 K193 T194 S195 N240 K241 A242 A244 K248	T4 H5 T7 P8 K9 N10 F11 G12 G14 Q15 Q18 P19 R21 L23 S24 R25 Y26 K28 E31 Y32 R34 R37 R45 L46 E77 K82 R85 D101 S103 P104 K105 P106 K110 Y111 G112 L113 H115 L119 K124 P136 L139 K160 A161 Q169 K170 T171 S172 N217 K218 A219 A221 K225
BS02	rna	G	Y55 V56 Q59 R60 K63 K181 R185	Y32 V33 Q36 R37 K40 K158 R162

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000470 maturation of LSU-rRNA
• GO:0002181 cytoplasmic translation
• GO:0006364 rRNA processing
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5h4p, PDBe:5h4p, PDBj:5h4p
• PDBsum: 5h4p
• PubMed: 28112732
• UniProt: P17076|RL8A_YEAST Large ribosomal subunit protein eL8A (Gene Name=RPL8A)