Structure of PDB 5gm6 Chain G

Receptor sequence

>5gm6G (length=875) Species: 559292 (Saccharomyces cerevisiae S288C)

SHQLGGQYSIPQDLRENLQKEAARIGENEKDVLQEKMETRTVQNREDSYH
KRRFDMKFELNKDEKKERTLSMLLLKIKNGNTASRRTSMRILTDKAVTFG
PEMIFNRLLPILLDRSLEDQERHLMIKTIDRVLYQLGDLTKPYVHKILVV
AAPLLIDEDPMVRSTGQEIITNLSTVAGLKTILTVMRPDIENEDEYVRNV
TSRAAAVVAKALGVNQLLPFINAACHSRKSWKARHTGIKIVQQIGILLGI
GVLNHLTGLMSCIKDCLMDDHVPVRIVTAHTLSTLAENSYPYGIEVFNVV
LEPLWKGIRSHRGKVLSSFLKAVGSMIPLMDPEYAGYYTTEAMRIIRREF
DSPDDEMKKTILLVLQKCSAVESITPKFLREEIAPEFFQKFWVRRVALDR
PLNKVVTYTTVTLAKKLGCSYTIDKLLTPLRDEAEPFRTMAVHAVTRTVN
LLGTADLDERLETRLIDALLIAFQEQTNSDSIIFKGFGAVTVSLDIRMKP
FLAPIVSTILNHLKHKTPLVRQHAADLCAILIPVIKNCHEFEMLNKLNII
LYESLGEVYPEVLGSIINAMYCITSVMDLDKLQPPINQILPTLTPILRNK
HRKVEVNTIKFVGLIGKLAPTYAPPKEWMRICFELLELLKSTNKEIRRSA
NATFGFIAEAIGPHDVLVALLNNLKVQERQLRVCTAVAIGIVAKVCGPYN
VLPVIMNEYTTPETNVQNGVLKAMSFMFEYIGNMSKDYIYFITPLLEDAL
TDRDLVHRQTASNVITHLALNCSGTGHEDAFIHLMNLLIPNIFETSPHAI
MRILEGLEALSQALGPGLFMNYIWAGLFHPAKNVRKAFWRVYNNMYVMYQ
DAMVPFYPVTPDNNEEYIEELDLVL

3D structure

• PDB: 5gm6 Structure of a yeast activated spliceosome at 3.5 angstrom resolution
• Chain: G
• Resolution: 3.5 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	G	T178 R182 R186 T738 S892 P893 P926 A927 K928	T82 R86 R90 T642 S796 P797 P830 A831 K832
BS02	rna	G	Y64 H65 I252 E254 R259 E291 N295 R299 I342 V368 R408 P449 N499 K500 T503 Y504 H539 R543 R698 T738 K740 R744 N747 R775 R778 V783 K818 H894 R898	Y49 H50 I156 E158 R163 E195 N199 R203 I246 V272 R312 P353 N403 K404 T407 Y408 H443 R447 R602 T642 K644 R648 N651 R679 R682 V687 K722 H798 R802

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0005515 protein binding

Biological Process

• GO:0000245 spliceosomal complex assembly
• GO:0000398 mRNA splicing, via spliceosome
• GO:0006397 mRNA processing
• GO:0008380 RNA splicing
• GO:1903241 U2-type prespliceosome assembly

Cellular Component

• GO:0005634 nucleus
• GO:0005681 spliceosomal complex
• GO:0005684 U2-type spliceosomal complex
• GO:0005686 U2 snRNP
• GO:0071004 U2-type prespliceosome
• GO:0071013 catalytic step 2 spliceosome

External links

• PDB: RCSB:5gm6, PDBe:5gm6, PDBj:5gm6
• PDBsum: 5gm6
• PubMed: 27445306
• UniProt: P49955|SF3B1_YEAST U2 snRNP component HSH155 (Gene Name=HSH155)