Structure of PDB 5f9h Chain G

Receptor sequence

>5f9hG (length=653) Species: 9606 (Homo sapiens)

PFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQ
KGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVE
NNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFN
YLDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVI
ATVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRI
CKREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYN
DALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELES
VSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEG
NPKLSFLKPGILTGRILDAFKASGDHNILIATSVADEGIDIAQCNLVILY
EYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSI
LRLQTWDEAVFREKILHIQTHEKFIRDSKPVPDKENKKLLCRKCKALACY
TADVRVIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNC
SHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFD
PAE

3D structure

• PDB: 5f9h Structural basis for m7G recognition and 2'-O-methyl discrimination in capped RNAs by the innate immune receptor RIG-I.
• Chain: G
• Resolution: 3.1 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	G	I300 G326 T347 Q349 K379 Q380 H381 Q507 K508 E510 Q511 R546 K635 T636 R637 T662 G663 T697 S698 N720 K888 S906	I61 G87 T108 Q110 K140 Q141 H142 Q258 K259 E261 Q262 R297 K386 T387 R388 T413 G414 T432 S433 N455 K619 S637	PDBbind-CN: Kd=1.8nM
BS02	ZN	G	C810 C813 C864 C869	C541 C544 C595 C600
BS03	MG	G	T636 L639	T387 L390
BS04	GTP	G	H830 H847 F853 K858 K861 I875 K888	H561 H578 F584 K589 K592 I606 K619

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0005524 ATP binding

External links

• PDB: RCSB:5f9h, PDBe:5f9h, PDBj:5f9h
• PDBsum: 5f9h
• PubMed: 26733676
• UniProt: O95786|RIGI_HUMAN Antiviral innate immune response receptor RIG-I (Gene Name=RIGI)