Structure of PDB 5a8w Chain G

Receptor sequence

>5a8wG (length=548) Species: 145261 (Methanothermobacter wolfeii)

KKLFLKALKKKFEGEDPEEKSTNFYCFGGWEQSERKREFTEYAKKAAEKR
GGIPFYNPDIGVPLGQRKLMAYRVSGTDAYVEGDDLHFVNNAAIQQMVDD
IKRTVIVGMDTAHAVLEKRLGVEVTPETINEYMEVINHALPGGAVVQEHM
VEVHPGIVEDCYAKVFTGDDNLADELDKRILIDINKEFPEEQAEQLKSYI
GNRTYQVNRVPTIVVRACDGGTVSRWSAMQIGMSFISAYKLCAGEAAIAD
FSFAAKHADVIEMGTIMPARRARGPNEPGGVAFGTFADIVQASRVSDDPA
NVSLEVIAGAAALYDQVWLGSYMSGGVGFTQYATAAYTDDILDDFLYYGM
EYVEDKFGICGSEPTMDVVRDISTEVTLYSLEQYEEYPTLLEDHFGGSQR
AAVAAAAAGCSTAFATGNSNAGVNGWYLSQILHKEAHSRLGFYGYDLQDQ
CGASNSLSIRSDEGLIHELRGPNYPNYAMNVGHQPEYAGIAQAPHAARGD
AFCTNPLIKVAFADKDLSFDFTSPRKSIAKGALREFIPEGERDLIIPA

3D structure

• PDB: 5a8w Didehydroaspartate Modification in Methyl-Coenzyme M Reductase Catalyzing Methane Formation.
• Chain: G
• Resolution: 1.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Q151 Y336 G448
• Catalytic site (residue number reindexed from 1): Q147 Y332 G444
• Enzyme Commision number: 2.8.4.1: coenzyme-B sulfoethylthiotransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	COM	G	Y336 F446 Y447	Y332 F442 Y443
BS02	F43	G	V331 G332 F333 T334 Q335 Y336 G445 F446	V327 G328 F329 T330 Q331 Y332 G441 F442
BS03	TP7	G	R229 K260 H261	R225 K256 H257
BS04	F43	G	A148 V149 V150 Q151 M154 Q234 M237 A247	A144 V145 V146 Q147 M150 Q230 M233 A243
BS05	TP7	G	R274 M327 F333 F446 M483 N484 V485	R270 M323 F329 F442 M479 N480 V481

Gene Ontology

Molecular Function

• GO:0046872 metal ion binding
• GO:0050524 coenzyme-B sulfoethylthiotransferase activity

Biological Process

• GO:0015948 methanogenesis

External links

• PDB: RCSB:5a8w, PDBe:5a8w, PDBj:5a8w
• PDBsum: 5a8w
• PubMed: 27467699
• UniProt: H7CHY2