Structure of PDB 3wfr Chain G

Receptor sequence

>3wfrG (length=461) Species: 32630 (synthetic construct)

AAAALNFYLSYFDDVAKVLPREHYCFIVGGWVRDRILGEPVGYNIDVDFL
TTADPVELAKNFAKRIGGHFFVFEKRGFLIKRPTIASVVLHLPPYRYRFD
FSPLKGKDLEKALIEDLKERDFTANAIAVNLDDVLSITIVYDPTGGIKDL
EQGLLRPVSIENLKRDPVRVLRGFRIAIEKNLQLTEDFYEFVKEDPRIVL
KSAVERITHELFKIMKEKTAHKVIRELYEYGVLEAIIPEIGRLREVKDPL
DEHTLKTLEYLEQVIEDRAKYLSAELLENFGKKRVLGEFTDVELLKWGAL
FHDIGKPQTFATFYEHDKVGAQIVREIGERLRWGDEATEFVAKLVRHHLR
PFFLREAFKKGELKRRGMANFWRECGDIAPHLFLLSIADAMASGDEEEDI
KALMETIAELESFNRNEMKEEAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAA

3D structure

• PDB: 3wfr Translocation and rotation of tRNA during template-independent RNA polymerization by tRNA nucleotidyltransferase
• Chain: G
• Resolution: 3.501 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	G	G41 D60 F83 F85 I92 D112 R132 D133 N137 D182 R185 E221 R222 R361 D364 X473 X476	G29 D48 F71 F73 I80 D100 R120 D121 N125 D166 R169 E205 R206 R332 D335 X431 X434
BS02	CTP	G	G42 R45 D58 D60 R132 D133 N137 R185 R188 R191	G30 R33 D46 D48 R120 D121 N125 R169 R172 R175
BS03	POP	G	G42 R45 R188 R191	G30 R33 R172 R175
BS04	MG	G	D58 D60	D46 D48

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0016779 nucleotidyltransferase activity

Biological Process

• GO:0006396 RNA processing

External links

• PDB: RCSB:3wfr, PDBe:3wfr, PDBj:3wfr
• PDBsum: 3wfr
• PubMed: 24389024
• UniProt: O67911|CATNT_AQUAE CC-adding tRNA nucleotidyltransferase (Gene Name=aq_2158)