Structure of PDB 3gli Chain G

Receptor sequence

>3gliG (length=373) Species: 83333 (Escherichia coli K-12)

GLEVLFQGPHMSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHA
YLFSGTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDL
IEIDAASRTKVEDTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALL
KTLEEPPEHVKFLLATTDPQKLPVTILSRCLQFHLKALDVEQIRHQLEHI
LNEEHIAHEPRALQLLARAAEGSLRDALSLTDQAIASGDGQVSTQAVSAM
LGTLDDDQALSLVEAMVEANGERVMALINEAAARGIEWEALLVEMLGLLH
RIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLIGRKELP
YAPDRRMGVEMTLLRALAFHPRM

3D structure

• PDB: 3gli The mechanism of ATP-dependent primer-template recognition by a clamp loader complex.
• Chain: G
• Resolution: 3.5 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	G	T99 K100 V101 S132 H134	T109 K110 V111 S142 H144	PDBbind-CN: Kd=0.38uM
BS02	peptide	G	L327 Y328 T331 R355 L357 A358 F359 H360 P361 R362	L337 Y338 T341 R365 L367 A368 F369 H370 P371 R372
BS03	ADP	G	A7 W10 R11 P12 V19 G48 V49 G50 K51 T52 S53 L214 R215	A17 W20 R21 P22 V29 G58 V59 G60 K61 T62 S63 L224 R225
BS04	BEF	G	R47 G48 K51 E127 R215	R57 G58 K61 E137 R225
BS05	MG	G	T52 D126	T62 D136
BS06	ZN	G	C73 C76 C79	C83 C86 C89

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity

Biological Process

• GO:0006260 DNA replication

Cellular Component

• GO:0009360 DNA polymerase III complex

External links

• PDB: RCSB:3gli, PDBe:3gli, PDBj:3gli
• PDBsum: 3gli
• PubMed: 19450514
• UniProt: P06710|DPO3X_ECOLI DNA polymerase III subunit tau (Gene Name=dnaX)