Structure of PDB 3eh8 Chain G

Receptor sequence

>3eh8G (length=254) Species: 162425 (Aspergillus nidulans) [Search protein sequence]

GSDLTYAYLVGLYEGDGYFSITKKGKYLTYELGIELSIKDVQLIYKIKKI
LGIGIVSFRKRNEIEMVALRIRDKNHLKSKILPIFEKYPMFSNKQYDYLR
FRNALLSGIIYLEDLPDYTRSDEPLNSIESIINTSYFSAWLVGFIEAEGC
FSVYKLNKDDDYLIASFDIAQRDGDILISAIRKYLSFTTKVYLDKTNCSK
LKVTSVRSVENIIKFLQNAPVKLLGNKKLQYKLWLKQLRKISRYSEKIKI
PSNY

3D structure

PDB

3eh8 Optimization of in vivo activity of a bifunctional homing endonuclease and maturase reverses evolutionary degradation.

Chain

Resolution

2.7 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	G	K24 Y27 I55 R59 R61 R70 R72 K74 Y111 E148 C150 S152 Y154 D168 A170 Q171 R172 K200 K202 Q230	K24 Y27 I55 R59 R61 R70 R72 K74 Y111 E148 C150 S152 Y154 D168 A170 Q171 R172 K200 K202 Q230	PDBbind-CN: Kd=12nM
BS02	dna	G	G15 D16 G17 Y18 S20 T22 K23 K24 E35 L36 S37 I64 M66 K94 Y162 K190 Y192 V206 R243	G15 D16 G17 Y18 S20 T22 K23 K24 E35 L36 S37 I64 M66 K94 Y162 K190 Y192 V206 R243	PDBbind-CN: Kd=12nM
BS03	CA	G	D16 A147	D16 A147
BS04	CA	G	G15 E148	G15 E148

Gene Ontology

	Molecular Function
GO:0004519	endonuclease activity

View graph for
Molecular Function

External links

PDB	RCSB:3eh8, PDBe:3eh8, PDBj:3eh8
PDBsum	3eh8
PubMed	19103658
UniProt	P03880\|ANI1_EMEND Intron-encoded DNA endonuclease I-AniI (Gene Name=I-AniI)

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