Structure of PDB 8wwu Chain F

Receptor sequence

>8wwuF (length=488) Species: 1615674 (Pseudomonas lactis)

DFITKNNLWTNEQRDAADKVLAEIDSLGLEMIRLSWADQYGLLRGKSLTV
ASLKSAFKEGSEVAIGPFFFDLVSSMVFNLFTTAFEDELSGNPTVVMVPD
PTTFKVLPWADKTGWMLADLHWKSGEPFPLCPRGIMKKAVKSLSDEGYLF
KCGIELEWYLTKIVDRSLSPESLGAPGVQPDAIQVQPVAQGYSVLLEHHL
DQVDDIMSKVRKGLLELNLPLRSIEDEWAPSQMETTFDVMEGLEAADAAL
LIKSAIKQICSRHGYHATFMCKPAINGFFASGWHMHQSLVDKDTRKNLFI
PSEGEVLSPLGRAYAGGLLANGSAASSFTTPTVNGYRRRQPYSLAPDRRA
WAKDNKAAMVRVVSATGDPASRIENRIGEPGANPYLYMASQIVSGLDGIK
NKKDPGELQESPYDAQVPMLPTTLAEALDALEHDSELFRSCFGDTFIKYW
LQLRRSEWARFLDAEGAEAAEPTGAVTQWEQKEYFNLL

3D structure

• PDB: 8wwu Discovery of the 1-naphthylamine biodegradation pathway reveals a glutamine synthetase-like protein that catalyzes 1-naphthylamine glutamylation
• Chain: F
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 6.3.1.2: glutamine synthetase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ANP	F	G160 E162 R229 F244 D245 V246 H293 S295 R368 R379 E381	G153 E155 R222 F237 D238 V239 H286 S288 R361 R372 E374
BS02	MN	F	E164 E234 E241	E157 E227 E234
BS03	MN	F	E162 H291 E381	E155 H284 E374
BS04	MG	F	E162 E241	E155 E234

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003824 catalytic activity
• GO:0004356 glutamine synthetase activity
• GO:0016879 ligase activity, forming carbon-nitrogen bonds
• GO:0046872 metal ion binding

Biological Process

• GO:0006542 glutamine biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0016020 membrane

External links

• PDB: RCSB:8wwu, PDBe:8wwu, PDBj:8wwu
• PDBsum: 8wwu
• UniProt: A0A7Y1Q2L1