Structure of PDB 8w8o Chain F

Receptor sequence

>8w8oF (length=346) Species: 300852 (Thermus thermophilus HB8) [Search protein sequence]

SDPVRQYLHEIGQVPLLTLEEEVELARKVEEGMEAIKKLSEITGLDPDLI
REVVRAKILGSARVRHIPGLKETLDPKTVEEIDQKLKSLPKEHKRYLHIA
REGEAARQHLIEANLRLVVSIAKKYTGRGLSFLDLIQEGNQGLIRAVEKF
EYKRRFKFSTYATWWIRQAINRAIADQARTIRIPVHMVETINKLSRTARQ
LQQELGREPTYEEIAEAMGPGWDAKRVEETLKIAQEPVSLETPIGDEKDS
FYGDFIPDEHLPSPVDAATQSLLSEELEKALSKLSEREAMVLKLRKGLID
GREHTLEEVGAFFGVTRERIRQIENKALRKLKYHESRTRKLRDFLD

3D structure

PDB

8w8o Nanopore tweezers show fractional-nucleotide translocation in sequence-dependent pausing by RNA polymerase.

Chain

Resolution

2.512 Å

3D
structure

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Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	dna	F	I321 D326 S327	I244 D249 S250
BS02	dna	F	D79 V81 R82 L85 G89 N191 R193 L194 S197 K200 F209 K226 F227 E228 R231 F233 K234 S236 T237 Y238 W241	D2 V4 R5 L8 G12 N114 R116 L117 S120 K123 F132 K149 F150 E151 R154 F156 K157 S159 T160 Y161 W164
BS03	MG	F	A292 G296 W299	A215 G219 W222

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0016987	sigma factor activity
GO:0046872	metal ion binding

	Biological Process
GO:0006352	DNA-templated transcription initiation
GO:0006355	regulation of DNA-templated transcription
GO:0010468	regulation of gene expression
GO:2000142	regulation of DNA-templated transcription initiation

	Cellular Component
GO:0005737	cytoplasm

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:8w8o, PDBe:8w8o, PDBj:8w8o
PDBsum	8w8o
PubMed	38990947
UniProt	Q5SKW1

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