Structure of PDB 8vhp Chain F

Receptor sequence

>8vhpF (length=732) Species: 83333 (Escherichia coli K-12)

QNLLVTKRDGSTERINLDKIHRVLDAAAEGLHNVSISQVELRSHIQFYDG
IKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLE
GKYLVQNRVTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVST
FKISLPTPIMSGVRTPTRQFSSCVLIECGDSLDSINATSSAIVKYVSQRA
GIGINAGRIRALGSPIRGGEAFHTGCIPFYKHFQTAVKSCSQGGVRGGAA
TLFYPMWHLEVESLLVLKNNRGVEGNRVRHMDYGVQINKLMYTRLLKGED
ITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQRVKAVELFSLM
MQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLNDV
NDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPA
AKRGAMGRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLL
KASNELAKEQGACPWFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEAL
RESIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRGYVSIKKDGILRQ
VVPDYEHLHDAYELLWEMPGNDGYLQLVGIMQKFIDQSISANTNYDPSRF
PSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRD

3D structure

• PDB: 8vhp How ATP and dATP Act as Molecular Switches to Regulate Enzymatic Activity in the Prototypical Bacterial Class Ia Ribonucleotide Reductase.
• Chain: F
• Resolution: 2.608 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ATP	F	S249 C292 S293	S247 C290 S291
BS02	ATP	F	V7 K9 R10 E15 I17 N18 K21 I22 T55 H59 K91	V5 K7 R8 E13 I15 N16 K19 I20 T53 H57 K89
BS03	ATP	F	K9 K21 H59 F87 K91 F97	K7 K19 H57 F85 K89 F95
BS04	ATP	F	D232 S233 L234 R262 I268 R269 H275 F281	D230 S231 L232 R260 I266 R267 H273 F279
BS05	CDP	F	P208 T209 C225 G253 Q294 R298 N437 L438 C439 E441 L464 M620 S622 T624 S625	P206 T207 C223 G251 Q292 R296 N435 L436 C437 E439 L462 M618 S620 T622 S623

External links

• PDB: RCSB:8vhp, PDBe:8vhp, PDBj:8vhp
• PDBsum: 8vhp
• PubMed: 39164005
• UniProt: P00452|RIR1_ECOLI Ribonucleoside-diphosphate reductase 1 subunit alpha (Gene Name=nrdA)