Structure of PDB 8upp Chain F

Receptor sequence

>8uppF (length=327) Species: 197 (Campylobacter jejuni)

ITVYYDKDCDLNLIKSKKVAIIGFGSQGHAHAMNLRDNGVNVTIGLREGS
VSAVKAKNAGFEVMSVSEASKIADVIMILAPDEIQADIFNVEIKPNLSEG
KAIAFAHGFNIHYGQIVVPKGVDVIMIAPKAPGHTVRNEFTLGGGTPCLI
AIHQDESKNAKNLALSYASAIGGGRTGIIETTFKAETETDLFGEQAVLCG
GLSALIQAGFETLVEAGYEPEMAYFECLHEMKLIVDLIYQGGIADMRYSI
SNTAEYGDYITGPKIITEETKKAMKGVLKDIQNGVFAKDFILERRAGFAR
MHAERKNMNDSLIEKTGRNLRAMMPWI

3D structure

• PDB: 8upp Mapping of the Reaction Trajectory catalyzed by Class I Ketol-Acid Reductoisomerase
• Chain: F
• Resolution: 1.78 Å

Enzymatic activity

• Enzyme Commision number: 1.1.1.86: ketol-acid reductoisomerase (NADP(+)).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NDP	F	I252 S253	I250 S251
BS02	X9W	F	A133 P134 D192 E196	A131 P132 D190 E194
BS03	X9W	F	E232 I252 S253 A256	E230 I250 S251 A254
BS04	MG	F	D192 E196	D190 E194
BS05	NDP	F	F26 G27 S28 Q29 R49 S54 L81 A82 P83 D84 I90 H109	F24 G25 S26 Q27 R47 S52 L79 A80 P81 D82 I88 H107

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0004455 ketol-acid reductoisomerase activity
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0050661 NADP binding

Biological Process

• GO:0008652 amino acid biosynthetic process
• GO:0009082 branched-chain amino acid biosynthetic process
• GO:0009097 isoleucine biosynthetic process
• GO:0009099 L-valine biosynthetic process

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:8upp, PDBe:8upp, PDBj:8upp
• PDBsum: 8upp
• UniProt: Q9PHN5|ILVC_CAMJE Ketol-acid reductoisomerase (NADP(+)) (Gene Name=ilvC)