Structure of PDB 8pzy Chain F

Receptor sequence

>8pzyF (length=499) Species: 652611 (Pseudomonas aeruginosa PA14)

FQGGMEFLVKSVRPETLKTATLVLAVGEGRKLGASAKAVDDATGGAISAV
LKRGDLAGKVGQTLLLQSLPNLKAERVLLVGAGKERELGDRQYRKLASAV
LSTLKGLAGADAALALGDLAVKGRGAHAKARLLVETLADGLYVFDRYKSQ
KAEPLKLKKLTLLADKADSAAVEQGSKEAQAIANGMALTRDLGNLPPNVC
HPTFLGEQAKGLAKEFKSLKVEVLDEKKLRELGMGSFLAVAQGSDQPPRL
IILQYNGAKKDQAPHVLVGKGITFDTGGISLKPGLGMDEMKFDMCGAASV
FGTFRAVLELQLPINLVGLLACAENMPSGGATRPGDIVTTMSGQTVEILN
TDAEGRLVLCDALTYAERFKPQSVIDIATLTGACIVALGSNTSGLMGNNE
ALVRQLLKAGEFADDRAWQLPLFDEYQEQLDSPFADIANIGGPKAGTITA
GCFLSRFAKKYHWAHLDIAGTAWISGGKDKGATGRPVPLLTQYLLERAK

3D structure

• PDB: 8pzy Unveiling the Catalytic Mechanism of a Processive Metalloaminopeptidase.
• Chain: F
• Resolution: 1.97 Å

Enzymatic activity

• Enzyme Commision number: 3.4.11.1: leucyl aminopeptidase.
  3.4.11.10: bacterial leucyl aminopeptidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BCT	F	A370 G372 R373 L397	A353 G355 R356 L380
BS02	P03	F	V43 G44 G46 R47 K48 L49	V26 G27 G29 R30 K31 L32
BS03	MN	F	K287 D292 D310 E371	K270 D275 D293 E354
BS04	MN	F	D292 K299 D369 E371	D275 K282 D352 E354

Gene Ontology

Molecular Function

• GO:0004177 aminopeptidase activity
• GO:0008235 metalloexopeptidase activity
• GO:0030145 manganese ion binding
• GO:0046872 metal ion binding
• GO:0070006 metalloaminopeptidase activity

Biological Process

• GO:0006508 proteolysis
• GO:0019538 protein metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:8pzy, PDBe:8pzy, PDBj:8pzy
• PDBsum: 8pzy
• PubMed: 37924287
• UniProt: Q02RY8|AMPA_PSEAB Probable cytosol aminopeptidase (Gene Name=pepA)