Structure of PDB 8j4z Chain F

Receptor sequence

>8j4zF (length=541) Species: 9606 (Homo sapiens)

YHGDSVASLGTQPDLGSALYQENYKQMKALVNQLHERVEHIKLGGGEKAR
ALHISRGKLLPRERIDNLIDPGSPFLELSQFAGYQLYDNEEVPGGGIITG
IGRVSGVECMIIANDATVKGGAYYPVTVKKQLRAQEIAMQNRLPCIYLVD
SGGAYLPRQADVFPDRDHFGRTFYNQAIMSSKNIAQIAVVMGSCTAGGAY
VPAMADENIIVRKQGTIFLAGPPLVKAATGEEVSAEDLGGADLHCRKSGV
SDHWALDDHHALHLTRKVVRNLNYQKKLDVTIEPSEEPLFPADELYGIVG
ANLKRSFDVREVIARIVDGSRFTEFKAFYGDTLVTGFARIFGYPVGIVGN
NGVLFSESAKKGTHFVQLCCQRNIPLLFLQNITGFMVGREYEAEGIAKDG
AKMVAAVACAQVPKITLIIGGSYGAGNYGMCGRAYSPRFLYIWPNARISV
MGGEQAANVLATITKDQRAREGKQFSSADEAALKEPIIKKFEEEGNPYYS
SARVWDDGIIDPADTRLVLGLSFSAALNAPIEKTDFGIFRM

3D structure

• PDB: 8j4z Human 3-methylcrotonyl-CoA carboxylase in BCCP-CTS state with substrate
• Chain: F
• Resolution: 2.73 Å

Enzymatic activity

• Enzyme Commision number: 6.4.1.4: methylcrotonoyl-CoA carboxylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BTI	F	L241 L246	L219 L224
BS02	TW3	F	I485 Q489	I463 Q467
BS03	TW3	F	R78 K141 G142 A144 G174 G175 A176 Y177 F191 T217 A218 G219	R56 K119 G120 A122 G152 G153 A154 Y155 F169 T195 A196 G197
BS04	BTI	F	G406 F407 Y445 A447	G384 F385 Y423 A425

Gene Ontology

Molecular Function

• GO:0004485 methylcrotonoyl-CoA carboxylase activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016874 ligase activity

Biological Process

• GO:0006552 L-leucine catabolic process
• GO:0009083 branched-chain amino acid catabolic process
• GO:0015936 coenzyme A metabolic process

Cellular Component

• GO:0005739 mitochondrion
• GO:0005759 mitochondrial matrix
• GO:0005829 cytosol
• GO:1905202 methylcrotonoyl-CoA carboxylase complex

External links

• PDB: RCSB:8j4z, PDBe:8j4z, PDBj:8j4z
• PDBsum: 8j4z
• UniProt: Q9HCC0|MCCB_HUMAN Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (Gene Name=MCCC2)