Structure of PDB 8gri Chain F

Receptor sequence

>8griF (length=482) Species: 285500 (Streptomyces luteocolor)

QTDVIVVGNGVLGLSVGVEIARTRPDVRVTLLGKPARQYGATPAAGAMLG
AFGEVTAHALASEHGRKKHALAVQAQRLWPEWIESLEATGTAADGRIKTA
DDTVVLLNTVGHSALDDANFAAVLTALKEANAPHEEIAVESVDWIDPDPN
SRPLRALHIEGEGSVDSGILLAALERSFLQAGGRLHPVDATEIRASHGRV
EGVVTDDGDFLPAGHVVVAAGARSQRLVAALPGLAHRIPRIYDGVGVSAL
VDTWDGSGPATVLRTSNRAFACGLHLVPRAGGSVYIGATNAVCLEPRGAA
SIAETVFLFNCATHQLHRGLNGSELRKVQVGSRPAPIDGFPLIGGTSVEG
LWMLSGTYRDGLHMSPLLARHVVSLMDGGTGVDGLREFRPERDLISAWSR
EEILDDVVRHTMATGYEFPWRLPLEWPHMMETFLQGPFAELADRLSDTYT
PPADLMTAIMFSEREQQDELIAYYADVHREWH

3D structure

• PDB: 8gri N-Formimidoylation/-iminoacetylation modification in aminoglycosides requires FAD-dependent and ligand-protein NOS bridge dual chemistry.
• Chain: F
• Resolution: 2.365 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	F	V16 G17 N18 G19 V20 L21 G42 R46 Q47 A50 T51 A54 A56 M57 D198 A199 A229 G230 R232 G340 R342 T366 Y367 R368 D369 G370 L371 H372	V7 G8 N9 G10 V11 L12 G33 R37 Q38 A41 T42 A45 A47 M48 D189 A190 A220 G221 R223 G331 R333 T357 Y358 R359 D360 G361 L362 H363
BS02	GLY	F	H284 R342 R368	H275 R333 R359
BS03	I55	F	X281 N299 S310 T423 T466 F470	X272 N290 S301 T414 T457 F461

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0016491 oxidoreductase activity

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:8gri, PDBe:8gri, PDBj:8gri
• PDBsum: 8gri
• PubMed: 37137912
• UniProt: A0A125SZC1