Structure of PDB 8eyk Chain F

Receptor sequence

>8eykF (length=1070) Species: 9606 (Homo sapiens)

SAAIYNIDTSSESPDHYLVDHTLDGRVLFPATGYLSIVWKTLARALGLGV
EQLPVVFEDVVLHQATILPKTGTVSLEVRLLEASRAFEVSENGNLVVSGK
VYQWDDPDPRLFDHPESPTPNPTEPLFLAQAEVYKELRLRGYDYGPHFQG
ILEASLEGDSGRLLWKDNWVSFMDTMLQMSILGSAKHGLYLPTRVTAIHI
DPATHRQKLYTLQDKAQVADVVVSRWLRVTVAGGVHISGLHTESAPRRQQ
EQQVPILEKFCFTPHTEGLLDSPALKACLDTAVENMPSLKMKVVEHLYSR
IPGLLSPHPLLQLSYTLLVCNPASALSNMVAALREGGFLLLHTLDAWESL
FSRVSLRLVGLKKLFLCRRPTPQDSPIFLPVDDTSFRWVESLKGILADED
SSRPVWLKAINCATSGVVGLVNCLRREPGGNRLRCVLLSNLSSTSHVPEV
DPGSAELQKVLQGDLVMNVYRDGAWGAFRHFLLEEDKPEEPTAHAFVSTL
TRGDLSSIRWVCSSLRHAQPTCPGAQLCTVYYASLNFRDIMLATGKLSPD
AIPGKWTSQDSLLGMEFSGRDASGKRVMGLVPAKGLATSVLLSPDFLWDV
PSNWTLEEAASVPVVYSTAYYALVVRGRVRPGETLLIHSGSGGVGQAAIA
IALSLGCRVFTTVGSAEKRAYLQARFPQLDSTSFANSRDTSFEQHVLWHT
GGKGVDLVLNSLAEEKLQASVRCLATHGRFLEIGKFDLSQNHPLGMAIFL
KNVTFHGVLLDAFFNEADWREVWALVQAGIRDGVVRPLKCTVFHGAQVED
AFRYMAQGKHIGKVVVQVLAEEPEAVLKKLMSAISKTFCPAHKSYIIAGG
LGGFGLELAQWLIQRGVQKLVLTSRSGIRTGYQAKQVRRWRRQGVQVQVS
TSNISSLEGARGLIAEAAQLGPVGGVFNLAVVLRDGLLENQTPEFFQDVC
KPKYSGTLNLDRVTREACPELDYFVVFSSVSCGRGNAGQSNYGFANSAME
RICEKRRHEGLPGLAVQWGAIGDVGILVETMSTNDTIVSGTLPQRMASCL
EVLDLFLNQPHMVLSSFVLA

3D structure

• PDB: 8eyk Atomic model for core modifying region of human fatty acid synthase in complex with Denifanstat.
• Chain: F
• Resolution: 2.7 Å

Enzymatic activity

• Enzyme Commision number: 1.1.1.100: 3-oxoacyl-[acyl-carrier-protein] reductase.
  1.3.1.39: enoyl-[acyl-carrier-protein] reductase (NADPH, Re-specific).
  2.3.1.38: [acyl-carrier-protein] S-acetyltransferase.
  2.3.1.39: [acyl-carrier-protein] S-malonyltransferase.
  2.3.1.41: beta-ketoacyl-[acyl-carrier-protein] synthase I.
  2.3.1.85: fatty-acid synthase system.
  3.1.2.14: oleoyl-[acyl-carrier-protein] hydrolase.
  4.2.1.59: 3-hydroxyacyl-[acyl-carrier-protein] dehydratase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	X5O	F	P1264 L1975 S2021 S2023 R2026 G2027 N2028 Q2031 L2069 S2081 T2083	P309 L933 S979 S981 R984 G985 N986 Q989 L1027 S1039 T1041
BS02	NDP	F	F1573 T1654 S1675 G1678 G1679 V1680 K1704 S1723 R1724 I1769 V1794 G1846 H1848	F537 T618 S639 G642 G643 V644 K668 S687 R688 I733 V758 G808 H810
BS03	NDP	F	G1895 T1915 S1916 R1917 N1945 L1971 A1972 V1973 K1995 F2019 Y2034 W2060 G2061 I2063 G2067 I2068	G853 T873 S874 R875 N903 L929 A930 V931 K953 F977 Y992 W1018 G1019 I1021 G1025 I1026

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0031177 phosphopantetheine binding

Biological Process

• GO:0009058 biosynthetic process

External links

• PDB: RCSB:8eyk, PDBe:8eyk, PDBj:8eyk
• PDBsum: 8eyk
• PubMed: 37308485
• UniProt: P49327|FAS_HUMAN Fatty acid synthase (Gene Name=FASN)