Structure of PDB 8e9h Chain F

Receptor sequence

>8e9hF (length=436) Species: 246196 (Mycolicibacterium smegmatis MC2 155)

TPLTPVLSRFWDEPEPWTLETYRRHDGYQGLQRALSMGPDDVIAFVKDSG
LRGRGGAGFPTGTKWSFIPQERGDQPAGGPAAKPHYLVINADESEPGTCK
DIPLLLTTPHFLVEGAIIAAYAIRARHAFIYVRGEVLPVLRRLQAAVAEA
YAAGYLGTDIMGSGFDLDLIVHAGAGAYICGEETALLDSLEGRRGQPRLR
PPFPAVAGLYACPTVVNNVESIASVPPIMVNGVDWFRSMGSEKSPGFTLY
SLSGHVTRPGQYEAPLGITLRELLEYAGGVRAGHQLKFWTPGGSSTPLLT
AEHLDVPLDYEGMASVGSMLGTKALQIFDETTCVVRAVRRWTQFYAHESC
GKCTPCREGTYWLAQIYARLENGAGTEADIDKLLDISDNIFGKSFCALGD
GAASPIMSSIKHFRDEYVAHLDGGCPFDPHASTLMA

3D structure

• PDB: 8e9h Structure of mycobacterial respiratory complex I.
• Chain: F
• Resolution: 2.7 Å

Enzymatic activity

• Enzyme Commision number: 7.1.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FMN	F	G54 R55 G56 A58 K65 N91 D93 Y179 G182 E183 E184 N218 N219 S222 L399	G53 R54 G55 A57 K64 N90 D92 Y178 G181 E182 E183 N217 N218 S221 L398
BS02	ZN	F	C334 E372 H421 C426	C333 E371 H420 C425
BS03	SF4	F	P198 S350 C351 G352 K353 C354 C357 S395 F396 C397 L399 G400	P197 S349 C350 G351 K352 C353 C356 S394 F395 C396 L398 G399

Gene Ontology

Molecular Function

• GO:0003954 NADH dehydrogenase activity
• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0010181 FMN binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0048038 quinone binding
• GO:0051287 NAD binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0045333 cellular respiration
• GO:1902600 proton transmembrane transport

Cellular Component

• GO:0045271 respiratory chain complex I

External links

• PDB: RCSB:8e9h, PDBe:8e9h, PDBj:8e9h
• PDBsum: 8e9h
• PubMed: 36952383
• UniProt: A0QU31