Structure of PDB 8e9g Chain F

Receptor sequence

>8e9gF (length=435) Species: 246196 (Mycolicibacterium smegmatis MC2 155)

TPLTPVLSRFWDEPEPWTLETYRRHDGYQGLQRALSMGPDDVIAFVKDSG
LRGRGGAGFPTGTKWSFIPQERGDQPAGGPAAKPHYLVINADESEPGTCK
DIPLLLTTPHFLVEGAIIAAYAIRARHAFIYVRGEVLPVLRRLQAAVAEA
YAAGYLGTDIMGSGFDLDLIVHAGAGAYICGEETALLDSLEGRRGQPRLR
PPFPAVAGLYACPTVVNNVESIASVPPIMVNGVDWFRSMGSEKSPGFTLY
SLSGHVTRPGQYEAPLGITLRELLEYAGGVRAGHQLKFWTPGGSSTPLLT
AEHLDVPLDYEGMASVGSMLGTKALQIFDETTCVVRAVRRWTQFYAHESC
GKCTPCREGTYWLAQIYARLENGAGTEADIDKLLDISDNIFGKSFCALGD
GAASPIMSSIKHFRDEYVAHLDGGCPFDPHASTLM

3D structure

• PDB: 8e9g Structure of mycobacterial respiratory complex I.
• Chain: F
• Resolution: 2.6 Å

Enzymatic activity

• Enzyme Commision number: 7.1.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	F	C334 E372 H421 C426	C333 E371 H420 C425
BS02	SF4	F	I180 P198 S350 C351 G352 K353 C354 C357 S395 F396 C397 L399 G400	I179 P197 S349 C350 G351 K352 C353 C356 S394 F395 C396 L398 G399
BS03	FMN	F	G54 R55 G56 K65 N91 D93 Y179 G182 E183 N218 N219 L399	G53 R54 G55 K64 N90 D92 Y178 G181 E182 N217 N218 L398

Gene Ontology

Molecular Function

• GO:0003954 NADH dehydrogenase activity
• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0010181 FMN binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0048038 quinone binding
• GO:0051287 NAD binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0045333 cellular respiration
• GO:1902600 proton transmembrane transport

Cellular Component

• GO:0045271 respiratory chain complex I

External links

• PDB: RCSB:8e9g, PDBe:8e9g, PDBj:8e9g
• PDBsum: 8e9g
• PubMed: 36952383
• UniProt: A0QU31