Structure of PDB 8b9z Chain F

Receptor sequence

>8b9zF (length=441) Species: 7227 (Drosophila melanogaster)

PPPGTPPPQTKTKFGPLADEDRIFTNLYGRHDWRLKGALKRGDWYKTKEI
VLKGADWIVNEIKTSGLRGRGGAGFPSGMKWSFMNKPGDGRPKYLVVNAD
EGEPGTCKDREIMRHDPHKLVEGCLIAGRAMGAQAAYIYIRGEFYNEASN
MQLAIAEAYQAGLIGKNACGTGYDFDVFMHRGAGAYICGEETALIESLEG
KQGKPRLKPPFPADVGVFGCPTTVTNVETVAVAPTICRRGGVWFASFGRT
RNSGTKLFNISGHVNRPCTVEEEMSIPLKELIERHCGGVTGGWDNLLGVI
PGGSSTPIIPKNVCDDVIMDFDGLIAAQTSLGTAAIIVMDKSTDVIKAIA
RLISFYKHESCGQCTPCREGIGWMNKIMTRFVKGDAQPAEIDMLWEISKQ
IEGHTICALGDGAAWPVQGLIRHFRPEIEKRMQLHAKRVSN

3D structure

• PDB: 8b9z Cryo-EM structures of mitochondrial respiratory complex I from Drosophila melanogaster.
• Chain: F
• Resolution: 3.28 Å

Enzymatic activity

• Enzyme Commision number: 7.1.1.2: NADH:ubiquinone reductase (H(+)-translocating).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FMN	F	G102 G104 K113 N131 D133 G222 E224 N259 A441 L442	G69 G71 K80 N98 D100 G189 E191 N226 A408 L409
BS02	SF4	F	P238 C394 G395 Q396 C397 C400 T438 C440 L442	P205 C361 G362 Q363 C364 C367 T405 C407 L409

Gene Ontology

Molecular Function

• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0010181 FMN binding
• GO:0046872 metal ion binding
• GO:0051287 NAD binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0006120 mitochondrial electron transport, NADH to ubiquinone
• GO:1902600 proton transmembrane transport

Cellular Component

• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane
• GO:0045271 respiratory chain complex I

External links

• PDB: RCSB:8b9z, PDBe:8b9z, PDBj:8b9z
• PDBsum: 8b9z
• PubMed: 36622099
• UniProt: Q9VMI3