Structure of PDB 8acw Chain F

Receptor sequence

>8acwF (length=407) Species: 666 (Vibrio cholerae)

MSTIIFGVVMFTLIILALVLVILFAKSKLVPTGDITISINGDPEKAIVTQ
PGGKLLTALAGAGVFVSSACGGGGSCGQCRVKIKSGGGDILPTELDHISK
GEAREGERLACQVAVKADMDLELPEEIFGVKKWECTVISNDNKATFIKEL
KLAIPDGESVPFRAGGYIQIEAPAHHVKYADFDVPEKYRGDWDKFNLFRY
ESKVDEPIIRAYSMANYPEEFGIIMLNVRIATPPPNNPNVPPGQMSSYIW
SLKAGDKCTISGPFGEFFAKDTDAEMVFIGGGAGMAPMRSHIFDQLKRLK
SKRKMSYWYGARSKREMFYVEDFDGLAAENDNFVWHCALSDPQPEDNWTG
YTGFIHNVLYENYLKDHEAPEDCEYYMCGPPMMNAAVINMLKNLGVEEEN
ILLDDFG

3D structure

• PDB: 8acw Conformational coupling of redox-driven Na + -translocation in Vibrio cholerae NADH:quinone oxidoreductase.
• Chain: F
• Resolution: 3.4 Å

Enzymatic activity

• Enzyme Commision number: 7.2.1.1: NADH:ubiquinone reductase (Na(+)-transporting).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	F	Y167 R210 A211 Y212 S213 N227 R229 A231 T232 V240 P241 P242 G243 Q244 M245 F406	Y167 R210 A211 Y212 S213 N227 R229 A231 T232 V240 P241 P242 G243 Q244 M245 F406
BS02	FES	F	C70 G71 G74 S75 C76 G77 C79 C111	C70 G71 G74 S75 C76 G77 C79 C111

Gene Ontology

Molecular Function

• GO:0009055 electron transfer activity
• GO:0016491 oxidoreductase activity
• GO:0016655 oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051537 2 iron, 2 sulfur cluster binding
• GO:0071949 FAD binding

Biological Process

• GO:0006814 sodium ion transport

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane

External links

• PDB: RCSB:8acw, PDBe:8acw, PDBj:8acw
• PDBsum: 8acw
• PubMed: 37710014
• UniProt: A5F5Y4|NQRF_VIBC3 Na(+)-translocating NADH-quinone reductase subunit F (Gene Name=nqrF)