Structure of PDB 8a1t Chain F

Receptor sequence

>8a1tF (length=405) Species: 666 (Vibrio cholerae)

STIIFGVVMFTLIILALVLVILFAKSKLVPTGDITISINGDPEKAIVTQP
GGKLLTALAGAGVFVSSACGGGGSCGQCRVKIKSGGGDILPTELDHISKG
EAREGERLACQVAVKADMDLELPEEIFGVKKWECTVISNDNKATFIKELK
LAIPDGESVPFRAGGYIQIEAPAHHVKYADFDVPEKYRGDWDKFNLFRYE
SKVDEPIIRAYSMANYPEEFGIIMLNVRIATPPPNNPNVPPGQMSSYIWS
LKAGDKCTISGPFGEFFAKDTDAEMVFIGGGAGMAPMRSHIFDQLKRLKS
KRKMSYWYGARSKREMFYVEDFDGLAAENDNFVWHCALSDPQPEDNWTGY
TGFIHNVLYENYLKDHEAPEDCEYYMCGPPMMNAAVINMLKNLGVEEENI
LLDDF

3D structure

• PDB: 8a1t Conformational coupling of redox-driven Na + -translocation in Vibrio cholerae NADH:quinone oxidoreductase.
• Chain: F
• Resolution: 3.37 Å

Enzymatic activity

• Enzyme Commision number: 7.2.1.1: NADH:ubiquinone reductase (Na(+)-transporting).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	F	Y167 A211 Y212 S213 N227 R229 A231 T232 V240 P241 P242 G243 Q244 F406	Y166 A210 Y211 S212 N226 R228 A230 T231 V239 P240 P241 G242 Q243 F405
BS02	FES	F	S68 C70 G71 G74 S75 C76 G77 C79 C111	S67 C69 G70 G73 S74 C75 G76 C78 C110

Gene Ontology

Molecular Function

• GO:0009055 electron transfer activity
• GO:0016491 oxidoreductase activity
• GO:0016655 oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051537 2 iron, 2 sulfur cluster binding
• GO:0071949 FAD binding

Biological Process

• GO:0006814 sodium ion transport

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane

External links

• PDB: RCSB:8a1t, PDBe:8a1t, PDBj:8a1t
• PDBsum: 8a1t
• PubMed: 37710014
• UniProt: A5F5Y4|NQRF_VIBC3 Na(+)-translocating NADH-quinone reductase subunit F (Gene Name=nqrF)