Structure of PDB 7xk5 Chain F

Receptor sequence

>7xk5F (length=408) Species: 345073 (Vibrio cholerae O395)

MSTIIFGVVMFTLIILALVLVILFAKSKLVPTGDITISINGDPEKAIVTQ
PGGKLLTALAGAGVFVSSACGGGGSCGQCRVKIKSGGGDILPTELDHISK
GEAREGERLACQVAVKADMDLELPEEIFGVKKWECTVISNDNKATFIKEL
KLAIPDGESVPFRAGGYIQIEAPAHHVKYADFDVPEKYRGDWDKFNLFRY
ESKVDEPIIRAYSMANYPEEFGIIMLNVRIATPPPNNPNVPPGQMSSYIW
SLKAGDKCTISGPFGEFFAKDTDAEMVFIGGGAGMAPMRSHIFDQLKRLK
SKRKMSYWYGARSKREMFYVEDFDGLAAENDNFVWHCALSDPQPEDNWTG
YTGFIHNVLYENYLKDHEAPEDCEYYMCGPPMMNAAVINMLKNLGVEEEN
ILLDDFGG

3D structure

• PDB: 7xk5 Cryo-EM structures of Na + -pumping NADH-ubiquinone oxidoreductase from Vibrio cholerae.
• Chain: F
• Resolution: 3.1 Å

Enzymatic activity

• Enzyme Commision number: 7.2.1.1: NADH:ubiquinone reductase (Na(+)-transporting).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FES	F	S68 A69 C70 G74 S75 C76 G77 Q78 C79	S68 A69 C70 G74 S75 C76 G77 Q78 C79
BS02	FAD	F	R210 A211 Y212 S213 V228 R229 A231 T232 V240 P241 G243 Q244 M245 F406 G407	R210 A211 Y212 S213 V228 R229 A231 T232 V240 P241 G243 Q244 M245 F406 G407

Gene Ontology

Molecular Function

• GO:0009055 electron transfer activity
• GO:0016491 oxidoreductase activity
• GO:0016655 oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051537 2 iron, 2 sulfur cluster binding
• GO:0071949 FAD binding

Biological Process

• GO:0006814 sodium ion transport

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane

External links

• PDB: RCSB:7xk5, PDBe:7xk5, PDBj:7xk5
• PDBsum: 7xk5
• PubMed: 35882843
• UniProt: A5F5Y4|NQRF_VIBC3 Na(+)-translocating NADH-quinone reductase subunit F (Gene Name=nqrF)