Structure of PDB 7w5y Chain F

Receptor sequence

>7w5yF (length=496) Species: 83333 (Escherichia coli K-12) [Search protein sequence]

AAAQVLSSVESEIGRTTDPVRMYMREMGTVELLTREGEIDIAKRIEDGIN
QVQCSVAEYPEAITYLLEQYDRVEAEEARLSDLITGFVDPNAENSIDPEL
AREKFAELRAQYVVTRDTIKAKGRSHATAQEEILKLSEVFKQFRLVPKQF
DYLVNSMRVMMDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWF
NAAIAMNKPWSEKLHDVSEEVHRALQKLQQIEEETGLTIEQVKDINRRMS
IGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAV
DKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRI
SRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPSMETPIGDDEDS
HLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGIDM
NTDYTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD

3D structure

PDB

7w5y Structural basis of three different transcription activation strategies adopted by a single regulator SoxS.

Chain

Resolution

4.2 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	F	R99 M102 L110 N383 R385 L386 K392 K418 E420 R423 Y425 K426 S428 T429 Y430 W433 W434 Q437 P453 H455 E585 R586	R21 M24 L32 N267 R269 L270 K276 K302 E304 R307 Y309 K310 S312 T313 Y314 W317 W318 Q321 P337 H339 E468 R469
BS02	dna	F	N396 E458 N461 E503 I511 D516 T572 L573 E574 E585 R588	N280 E342 N345 E387 I394 D399 T455 L456 E457 E468 R471

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003700	DNA-binding transcription factor activity
GO:0005515	protein binding
GO:0016987	sigma factor activity

	Biological Process
GO:0006351	DNA-templated transcription
GO:0006352	DNA-templated transcription initiation
GO:0006355	regulation of DNA-templated transcription
GO:0009408	response to heat
GO:0010468	regulation of gene expression
GO:0045892	negative regulation of DNA-templated transcription
GO:2000142	regulation of DNA-templated transcription initiation

	Cellular Component
GO:0000345	cytosolic DNA-directed RNA polymerase complex
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:1903865	sigma factor antagonist complex

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:7w5y, PDBe:7w5y, PDBj:7w5y
PDBsum	7w5y
PubMed	36243985
UniProt	P00579\|RPOD_ECOLI RNA polymerase sigma factor RpoD (Gene Name=rpoD)

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