Structure of PDB 7w5x Chain F

Receptor sequence

>7w5xF (length=495) Species: 83333 (Escherichia coli K-12) [Search protein sequence]

AAAQVLSSVESEIGRTTDPVRMYMREMGTVELLTREGEIDIAKRIEDGIN
QVQCSVAEYPEAITYLLEQYDRVEAEEARLSDLITGFVDPNAENSIDPEL
AREKFAELRAQYVVTRDTIKAKGRSHATAQEEILKLSEVFKQFRLVPKQF
DYLVNSMRVMMDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWF
NAAIAMNKPWSEKLHDVSEEVHRALQKLQQIEEETGLTIEQVKDINRRMS
IGEAKARRAKKEMVEANLRLVISIAKKYTRGLQFLDLIQEGNIGLMKAVD
KFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRIS
RQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISMETPIGDDEDS
HLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGIDM
NTDYTLEEVGKQFDVTRERIRQIEAKALRKLRPSRSEVLRSFLDD

3D structure

PDB

7w5x Structural basis of three different transcription activation strategies adopted by a single regulator SoxS.

Chain

Resolution

3.4 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	F	D96 R99 M102 G106 L110 N383 R385 L386 I388 K418 F419 E420 R423 Y425 K426 S428 T429 Y430 T432 W433 Q437 R451 P453 H455 T583 E585 R586	D18 R21 M24 G28 L32 N267 R269 L270 I272 K301 F302 E303 R306 Y308 K309 S311 T312 Y313 T315 W316 Q320 R334 P336 H338 T466 E468 R469
BS02	dna	F	Y394 R397 G398 E458 P510 I511 D513 R562 T572 L573 E585 R588	Y278 R280 G281 E341 P393 I394 D396 R445 T455 L456 E468 R471

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003700	DNA-binding transcription factor activity
GO:0005515	protein binding
GO:0016987	sigma factor activity

	Biological Process
GO:0006351	DNA-templated transcription
GO:0006352	DNA-templated transcription initiation
GO:0006355	regulation of DNA-templated transcription
GO:0009408	response to heat
GO:0010468	regulation of gene expression
GO:0045892	negative regulation of DNA-templated transcription
GO:2000142	regulation of DNA-templated transcription initiation

	Cellular Component
GO:0000345	cytosolic DNA-directed RNA polymerase complex
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:1903865	sigma factor antagonist complex

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Molecular Function

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Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:7w5x, PDBe:7w5x, PDBj:7w5x
PDBsum	7w5x
PubMed	36243985
UniProt	P00579\|RPOD_ECOLI RNA polymerase sigma factor RpoD (Gene Name=rpoD)

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