Structure of PDB 7uus Chain F

Receptor sequence

>7uusF (length=322) Species: 246196 (Mycolicibacterium smegmatis MC2 155)

ASVLWFQGGACSGNTMSFLNADEPNVVDLIVDFGLDLLWHPSLGLELGNN
AQKVFWDCAKGERPLDIFVFEGTVIEAPNGTGQMDMFAGRPMKDWVTDLA
GAAQIVVAIGDCACFGGIPAMEPNPSGSTGLQFHKREKGGFLGPDFRSKM
GLPVINVPGCPAHPDWITQILVALATGRAGDITLDDLHRPETFFKTFTQT
GCTRVQFFEYKQSTLSFGEGTRTGCLFYEFGCRGPMTHSPCNRILWNRQS
SKTRAGMPCLGCTEPEFPHFDLAPGTVFKTQKVSGMIPKEVPEGTDHLTY
MGLAAAARIAAPQWSKEDMFVV

3D structure

• PDB: 7uus Structural basis for bacterial energy extraction from atmospheric hydrogen.
• Chain: F
• Resolution: 8.0 Å

Enzymatic activity

• Enzyme Commision number: 1.12.99.6: hydrogenase (acceptor).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MQ9	F	K212 Q213 S214 F228 Y229 M287 Y301 M302 A306 R309	K211 Q212 S213 F227 Y228 M286 Y300 M301 A305 R308
BS02	F3S	F	A11 C12 G14 N15 E72 G111 C113 C161 P162	A10 C11 G13 N14 E71 G110 C112 C160 P161
BS03	F3S	F	T199 C242 W247 K253 C260 L261 G262 C263 T264	T198 C241 W246 K252 C259 L260 G261 C262 T263
BS04	F3S	F	Q200 C203 R205 V206 C226 L227 F228 C233 G235 P236 T254	Q199 C202 R204 V205 C225 L226 F227 C232 G234 P235 T253

Gene Ontology

Molecular Function

• GO:0008901 ferredoxin hydrogenase activity
• GO:0009055 electron transfer activity
• GO:0016491 oxidoreductase activity
• GO:0033748 hydrogenase (acceptor) activity
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051538 3 iron, 4 sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0009061 anaerobic respiration

Cellular Component

• GO:0009375 ferredoxin hydrogenase complex
• GO:0016020 membrane
• GO:0044569 [Ni-Fe] hydrogenase complex

External links

• PDB: RCSB:7uus, PDBe:7uus, PDBj:7uus
• PDBsum: 7uus
• PubMed: 36890228
• UniProt: A0QUM6