Structure of PDB 7t20 Chain F

Receptor sequence
>7t20F (length=447) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
KVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDFYTRPHRHIFTEMAR
LQESGSPIDLITLAESLERQGQLDSVGGFAYLAELSKNTPSAANISAYAD
IVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDLAESRVFKIAESRAN
KDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPS
DLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLA
SLSRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVR
SRARRIAREHGGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKE
LNVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVY
HENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQYDDE
3D structure
PDB7t20 Sequence of conformation changes of DnaB helicase during DNA unwinding and priming in Escherichia coli
ChainF
Resolution4.7 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.6.4.12: DNA helicase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna F N386 R387 N362 R363
BS02 ANP F P233 S234 G236 K237 T238 T239 E262 M263 R271 T282 Q384 G455 P209 S210 G212 K213 T214 T215 E238 M239 R247 T258 Q360 G431
BS03 MG F K237 T238 E262 K213 T214 E238
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003678 DNA helicase activity
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016787 hydrolase activity
GO:0016853 isomerase activity
GO:0016887 ATP hydrolysis activity
GO:0042802 identical protein binding
GO:0043139 5'-3' DNA helicase activity
Biological Process
GO:0006260 DNA replication
GO:0006268 DNA unwinding involved in DNA replication
GO:0006269 DNA replication, synthesis of primer
GO:0006270 DNA replication initiation
GO:0010212 response to ionizing radiation
GO:0031297 replication fork processing
GO:0032508 DNA duplex unwinding
Cellular Component
GO:0005829 cytosol
GO:0030894 replisome
GO:0033202 DNA helicase complex
GO:1990077 primosome complex
GO:1990100 DnaB-DnaC complex
GO:1990156 DnaB-DnaG complex
GO:1990158 DnaB-DnaC-DnaT-PriA-PriB complex
GO:1990159 DnaB-DnaC-DnaT-PriA-PriC complex
GO:1990160 DnaB-DnaC-Rep-PriC complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:7t20, PDBe:7t20, PDBj:7t20
PDBsum7t20
PubMed
UniProtP0ACB0|DNAB_ECOLI Replicative DNA helicase DnaB (Gene Name=dnaB)

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