Structure of PDB 7q4v Chain F

Receptor sequence

>7q4vF (length=470) Species: 931626 (Acetobacterium woodii DSM 1030)

NIDEYIGFDGYLALEKVLLTMSPVDVINEVKASGLRGRGGGGFPTGLKWQ
FAHDAVSEDGIKYVACNADEGDPGAFMDRSVLEGDPHAVIEAMAIAGYAV
GASKGYVYVRAEYPIAVNRLQIAIDQAKEYGILGENIFETDFSFDLEIRL
GAGAFVCGEETALMNSIEGKRGEPRPRPPFPANKGLFGKPTVLNNVETYA
NIPKIILNGAEWFASVGTEKSKGTKVFALGGKINNTGLLEIPMGTTLREI
IYEIGGGIPNGKAFKAAQTGGPSGGCLPESLLDTEIDYDNLIAAGSMMGS
GGLIVMDEDNCMVDVARFFLDFTQDESCGKCPPCRIGTKRMLEILERICD
GKGVEGDIERLEELAVGIKSSALCGLGQTAPNPVLSTIRFFRDEYEAHIR
DKKCPAGVCKHLLDFKINADTCKGCGICAKKCPADAISGEKKKPYNIDTS
KCIKCGACIEACPFGSISKA

3D structure

• PDB: 7q4v Molecular Basis of the Electron Bifurcation Mechanism in the [FeFe]-Hydrogenase Complex HydABC.
• Chain: F
• Resolution: 4.7 Å

Enzymatic activity

• Enzyme Commision number: 1.12.7.2: ferredoxin hydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	F	F544 C561 P562 I566 C581 K583 C584 G585 A586 C587	F415 C432 P433 I437 C452 K454 C455 G456 A457 C458
BS02	SF4	F	I546 C551 G553 C554 G555 I556 C557 Y574 C591 P592 I596	I417 C422 G424 C425 G426 I427 C428 Y445 C462 P463 I467
BS03	ZN	F	C440 H527 C533 C538	C311 H398 C404 C409
BS04	FMN	F	G166 G168 N196 G287 E288 E289 G504	G37 G39 N67 G158 E159 E160 G375
BS05	NAD	F	G169 F172 K177 R306 F309 M426	G40 F43 K48 R177 F180 M297
BS06	SF4	F	S456 C457 G458 K459 C460 C463 L502 C503	S327 C328 G329 K330 C331 C334 L373 C374

Gene Ontology

Molecular Function

• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0008901 ferredoxin hydrogenase activity
• GO:0010181 FMN binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:1902600 proton transmembrane transport

External links

• PDB: RCSB:7q4v, PDBe:7q4v, PDBj:7q4v
• PDBsum: 7q4v
• PubMed: 36811855
• UniProt: H6LFG4