Structure of PDB 7n9z Chain F

Receptor sequence

>7n9zF (length=658) Species: 562 (Escherichia coli)

MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYLWKEWLT
SVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYD
QIFTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTV
VGVILVNVSLGVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIG
TTLTGINFFVTILKMRAPGMTMFKMPVFTWASLCANVLIIASFPILTVTV
ALLTLDRYLGTHFFTNDMGGNMMMYINLIWAWGHPEVYILILPVFGVFSE
IAATFSRKRLFGYTSLVWATVCITVLSFIVWLHHFFTMGAGANVNAFFGI
TTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFSVGGMTGVL
LAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAAS
GAVLIALGILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFY
NFAVVPHVHERDAFWEMKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFST
IFGFAMIWHIWWLAIVGFAGMIITWIVKSFDEDVDYYVPVAEIEKLENQH
FDEITKAG

3D structure

• PDB: 7n9z Cryo-EM structures of Escherichia coli cytochrome bo 3 reveal bound phospholipids and ubiquinone-8 in a dynamic substrate binding site.
• Chain: F
• Resolution: 2.19 Å

Enzymatic activity

• Enzyme Commision number: 7.1.1.3: ubiquinol oxidase (H(+)-transporting).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	UQ8	F	T20 V67 R71 A74 D75 M78 I102 N157 L160	T20 V67 R71 A74 D75 M78 I102 N157 L160
BS02	HEO	F	V287 I291 H333 H334 T359 G360 F391 G395 G398 V399 L401 A402 D407 H411 L416 H419 F420 V423 R481	V287 I291 H333 H334 T359 G360 F391 G395 G398 V399 L401 A402 D407 H411 L416 H419 F420 V423 R481
BS03	HEM	F	A76 M79 R80 F103 H106 G107 M110 I111 G169 W170 F420 H421 I424 F468 R481 R482	A76 M79 R80 F103 H106 G107 M110 I111 G169 W170 F420 H421 I424 F468 R481 R482
BS04	CU	F	H284 H333 H334	H284 H333 H334

Gene Ontology

Molecular Function

• GO:0004129 cytochrome-c oxidase activity
• GO:0005507 copper ion binding
• GO:0005515 protein binding
• GO:0009055 electron transfer activity
• GO:0009486 cytochrome bo3 ubiquinol oxidase activity
• GO:0015078 proton transmembrane transporter activity
• GO:0015453 oxidoreduction-driven active transmembrane transporter activity
• GO:0016682 oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor
• GO:0020037 heme binding
• GO:0046872 metal ion binding
• GO:0048039 ubiquinone binding

Biological Process

• GO:0009060 aerobic respiration
• GO:0015990 electron transport coupled proton transport
• GO:0019646 aerobic electron transport chain
• GO:0022904 respiratory electron transport chain
• GO:1902600 proton transmembrane transport

Cellular Component

• GO:0005886 plasma membrane
• GO:0009319 cytochrome o ubiquinol oxidase complex
• GO:0016020 membrane

External links

• PDB: RCSB:7n9z, PDBe:7n9z, PDBj:7n9z
• PDBsum: 7n9z
• PubMed: 34417297
• UniProt: P0ABI8|CYOB_ECOLI Cytochrome bo(3) ubiquinol oxidase subunit 1 (Gene Name=cyoB)