Structure of PDB 7fs6 Chain F

Receptor sequence

>7fs6F (length=432) Species: 9606 (Homo sapiens)

ADVAQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSII
ATIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESF
AGSPLSYRPVAIALDTKGPPGLSEQDVRDLRFGVEHGVDIVFASFVRKAS
DVAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGI
EIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVA
NAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELR
RAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAA
VIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKL
RGFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS

3D structure

• PDB: 7fs6 Tuning liver pyruvate kinase activity up or down with a new class of allosteric modulators.
• Chain: F
• Resolution: 2.241 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.40: pyruvate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FBP	F	L443 T444 T445 T446 S449 W494 R501 G526 R528 P529 G530 S531 G532 Y533 T534	L332 T333 T334 T335 S338 W383 R390 G415 R417 P418 G419 S420 G421 Y422 T423
BS02	OXL	F	K282 E284 A305 G307 D308 T340	K171 E173 A194 G196 D197 T229
BS03	MG	F	E284 D308	E173 D197
BS04	Y3Z	F	H90 G307 D308 I311 T340 Q341	H80 G196 D197 I200 T229 Q230
BS05	Y3Z	F	H90 E344 I347 G375 K379	H80 E233 I236 G264 K268
BS06	Y3Z	F	F38 L39 L42 Y402 Q405 L406 E409	F28 L29 L32 Y291 Q294 L295 E298

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0004743 pyruvate kinase activity
• GO:0030955 potassium ion binding

Biological Process

• GO:0006096 glycolytic process

External links

• PDB: RCSB:7fs6, PDBe:7fs6, PDBj:7fs6
• PDBsum: 7fs6
• PubMed: 36753880
• UniProt: P30613|KPYR_HUMAN Pyruvate kinase PKLR (Gene Name=PKLR)