Structure of PDB 7frx Chain F

Receptor sequence

>7frxF (length=432) Species: 9606 (Homo sapiens)

ADVAQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSII
ATIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESF
AGSPLSYRPVAIALDTKGPPGLSEQDVRDLRFGVEHGVDIVFASFVRKAS
DVAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGI
EIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVA
NAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELR
RAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAA
VIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKL
RGFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS

3D structure

• PDB: 7frx Tuning liver pyruvate kinase activity up or down with a new class of allosteric modulators.
• Chain: F
• Resolution: 1.847 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.40: pyruvate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FBP	F	L443 T444 T445 T446 S449 W494 R501 G526 R528 P529 G530 S531 Y533 T534	L332 T333 T334 T335 S338 W383 R390 G415 R417 P418 G419 S420 Y422 T423
BS02	OXL	F	K282 E284 A305 G307 D308 T340	K171 E173 A194 G196 D197 T229
BS03	MG	F	E284 D308	E173 D197
BS04	O88	F	F38 L39 L42 Y402 L406 E409	F28 L29 L32 Y291 L295 E298

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0004743 pyruvate kinase activity
• GO:0030955 potassium ion binding

Biological Process

• GO:0006096 glycolytic process

External links

• PDB: RCSB:7frx, PDBe:7frx, PDBj:7frx
• PDBsum: 7frx
• PubMed: 36753880
• UniProt: P30613|KPYR_HUMAN Pyruvate kinase PKLR (Gene Name=PKLR)